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Literature summary extracted from

  • Watanabe, K.; Fujiwara, H.; Inui, K.; Suzuki, Y.
    Oligo-1,6-glucosidase from a thermophile, Bacillus thermoglucosidasius KP1006, was efficiently produced by combinatorial expression of GroEL in Escherichia coli (2002), Biotechnol. Appl. Biochem., 35, 35-43.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.10 GroEL heat shock protein of the Hsp60 team, GenBank accession number AB025944, activates 1.56fold, revives the thermally denatured enzyme, stabilizes and improve the recombinant expression level in Escherichia coli Parageobacillus thermoglucosidasius
3.2.1.10 GroES heat shock protein of the Hsp60 team, GenBank accession number AB025944, activates 1.44fold, revives the thermally denatured enzyme, stabilizes and improve the recombinant expression level in Escherichia coli Parageobacillus thermoglucosidasius

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.10 optimization of recombinant overproduction in Escherichia coli BL21(DE3) by coexpression with various Bacillus thermoglucosidarius chaperone proteins, i.e. Hsp60 team proteins GroES and GroEL, as well as Hsp70 team proteins GrpE, DnaK, and DnaJ, thereof the Hsp70 proteins have no effect on expression level, while the Hsp60 proteins, especially GroEL, increase the expression level by 3.8fold Parageobacillus thermoglucosidasius

General Stability

EC Number General Stability Organism
3.2.1.10 N-terminal domain alpha/beta motifs are probably recognized by the endogenous GroEL protein, leading to binding and stabilization Parageobacillus thermoglucosidasius

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.10 DnaJ the coexpression of the Hsp70 team chaperone protein leads to reduced activity Parageobacillus thermoglucosidasius
3.2.1.10 DnaK the coexpression of the Hsp70 team chaperone protein leads to reduced activity Parageobacillus thermoglucosidasius
3.2.1.10 GrpE the coexpression of the Hsp70 team chaperone protein leads to reduced activity Parageobacillus thermoglucosidasius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.10 66000
-
 x * 66000 Parageobacillus thermoglucosidasius

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Parageobacillus thermoglucosidasius
-
-
-
3.2.1.10 Parageobacillus thermoglucosidasius KP1006
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.10 thermally inactivated enzyme is revived by GroEL Parageobacillus thermoglucosidasius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.10 additional information
-
-
 Parageobacillus thermoglucosidasius
3.2.1.10 5.1
-
 purified recombinant enzyme + coexpressed DnaJ, DnaK, and GrpE Parageobacillus thermoglucosidasius
3.2.1.10 6.07
-
 purified recombinant enzyme Parageobacillus thermoglucosidasius
3.2.1.10 8.74
-
 purified recombinant enzyme + coexpressed GroES Parageobacillus thermoglucosidasius
3.2.1.10 9.46
-
 purified recombinant enzyme + coexpressed GroES and GroEL Parageobacillus thermoglucosidasius
3.2.1.10 9.48
-
 purified recombinant enzyme + coexpressed GroEL Parageobacillus thermoglucosidasius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Parageobacillus thermoglucosidasius 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Parageobacillus thermoglucosidasius KP1006 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.10 additional information N-terminal domain alpha/beta motifs are probably recognized by the endogenous GroEL protein, leading to binding and stabilization Parageobacillus thermoglucosidasius ?
-
 ?
3.2.1.10 additional information N-terminal domain alpha/beta motifs are probably recognized by the endogenous GroEL protein, leading to binding and stabilization Parageobacillus thermoglucosidasius KP1006 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.10 ? x * 66000 Parageobacillus thermoglucosidasius

Synonyms

EC Number Synonyms Comment Organism
3.2.1.10 More the enzyme belongs to the glycosyl hydrolase family 13 Parageobacillus thermoglucosidasius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.10 60
-
 assay at Parageobacillus thermoglucosidasius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.10 6.8
-
 assay at Parageobacillus thermoglucosidasius