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Literature summary extracted from

  • Kizaki, N.; Sawa, I.; Yano, M.; Yasohara, Y.; Hasegawa, J.
    Purification and characterization of a yeast carbonyl reductase for synthesis of optically active (R)-styrene oxide derivatives (2005), Biosci. Biotechnol. Biochem., 69, 79-86.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.184 synthesis semienzymatic production of (R)-3-styrene oxide Rhodotorula glutinis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.184 2,4-dinitrophenol complete inhibition at 1 mM Rhodotorula glutinis
1.1.1.184 Ag+ complete inhibition at 1 mM Rhodotorula glutinis
1.1.1.184 Diphenylhydantoin 68% inhibition at 0.5 mM Rhodotorula glutinis
1.1.1.184 DTNB complete inhibition at 1 mM Rhodotorula glutinis
1.1.1.184 Hg2+ complete inhibition at 1 mM Rhodotorula glutinis
1.1.1.184 iodoacetate 19% inhibition at 1 mM Rhodotorula glutinis
1.1.1.184 additional information not affected by EDTA and 1,10-phenanthroline at 1 mM Rhodotorula glutinis
1.1.1.184 NEM 30% inhibition at 1 mM Rhodotorula glutinis
1.1.1.184 p-chloromercuribenzoate 76% inhibition at 1 mM Rhodotorula glutinis
1.1.1.184 quercetin complete inhibition at 0.1 mM Rhodotorula glutinis
1.1.1.184 Zn2+ 43% inhibition at 1 mM Rhodotorula glutinis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.184 Fe2+ 14% activation at 1 mM Rhodotorula glutinis
1.1.1.184 Mn2+ 20% activation at 1 mM Rhodotorula glutinis
1.1.1.184 additional information enzyme is not affected by Mg2+, Ca2+ and Cu2+ at 1 mM Rhodotorula glutinis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 30000
-
1 * 30000, SDS-PAGE Rhodotorula glutinis
1.1.1.184 40000
-
gel filtration Rhodotorula glutinis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Rhodotorula glutinis
-
var. dairenensis, IFO415
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 487fold to homogeneity in a 4-step procedure Rhodotorula glutinis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.184 3 8 purified enzyme Rhodotorula glutinis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 2-bromo-aceto-2-chlorophenone + NADPH stereospecific enzyme Rhodotorula glutinis (R)-2-bromo-1-(2-chlorophenyl)ethanol + NADP+ product reacts spontaneously to the (R)-styrene oxide derivative in NaOH ?
1.1.1.184 2-bromo-aceto-3-chlorophenone + NADPH stereospecific enzyme Rhodotorula glutinis (R)-2-bromo-1-(3-chlorophenyl)ethanol + NADP+ product reacts spontaneously to the (R)-styrene oxide derivative in NaOH ?
1.1.1.184 2-bromo-aceto-4-chlorophenone + NADPH stereospecific enzyme Rhodotorula glutinis (R)-2-bromo-1-(4-chlorophenyl)ethanol + NADP+ product reacts spontaneously to the (R)-styrene oxide derivative in NaOH ?
1.1.1.184 2-bromo-acetophenone + NADPH i.e. phenacyl bromide, stereospecific enzyme Rhodotorula glutinis (R)-2-bromo-1-phenylethanol + NADP+ product reacts spontaneously to the (R)-styrene oxide derivative in NaOH ?
1.1.1.184 2-butanone + NADPH low activity Rhodotorula glutinis 2-butanol + NADP+
-
?
1.1.1.184 2-chloro-aceto-3-chlorophenone + NADPH i.e. 3-chlorophenacyl chloride Rhodotorula glutinis (R)-2-chloro-1-(3-chlorophenyl)ethanol + NADP+ product reacts spontaneously to the (R)-styrene oxide derivative in NaOH ?
1.1.1.184 2-chlorobenzaldehyde + NADPH + H+
-
Rhodotorula glutinis 2-chlorobenzylalcohol + NADP+
-
?
1.1.1.184 2-halo-acetophenone + NADPH i.e. phenacyl halide, stereospecific enzyme Rhodotorula glutinis (R)-2-halo-1-phenylethanol + NADP+ product reacts spontaneously to the (R)-styrene oxide derivative in NaOH ?
1.1.1.184 2-heptanone + NADPH
-
Rhodotorula glutinis 2-heptanol + NADP+
-
?
1.1.1.184 2-nitrobenzaldehyde + NADPH
-
Rhodotorula glutinis 2-nitrobenzylalcohol + NADP+
-
?
1.1.1.184 2-oxobutyric acid + NADPH low activity Rhodotorula glutinis 2-hydroxybutyric acid + NADP+
-
?
1.1.1.184 3-chlorobenzaldehyde + NADPH + H+
-
Rhodotorula glutinis 3-chlorobenzylalcohol + NADP+
-
?
1.1.1.184 3-nitroacetophenone + NADPH
-
Rhodotorula glutinis 1-(3-nitrophenyl)ethanol + NADP+
-
?
1.1.1.184 3-nitrobenzaldehyde + NADPH
-
Rhodotorula glutinis 3-nitrobenzylalcohol + NADP+
-
?
1.1.1.184 4-acetylpyridine + NADPH
-
Rhodotorula glutinis ? + NADP+
-
?
1.1.1.184 4-chlorobenzaldehyde + NADPH + H+
-
Rhodotorula glutinis 4-chlorobenzylalcohol + NADP+
-
?
1.1.1.184 4-nitrobenzaldehyde + NADPH
-
Rhodotorula glutinis 4-nitrobenzylalcohol + NADP+
-
?
1.1.1.184 acetone + NADPH
-
Rhodotorula glutinis 2-propanol + NADP+
-
?
1.1.1.184 benzaldehyde + NADPH
-
Rhodotorula glutinis benzylalcohol + NADP+
-
?
1.1.1.184 camphorquinone + NADPH
-
Rhodotorula glutinis ? + NADP+
-
?
1.1.1.184 cyclopentanone + NADPH low activity Rhodotorula glutinis cyclopentanol + NADP+
-
?
1.1.1.184 diacetyl + NADPH low activity Rhodotorula glutinis ? + NADP+
-
?
1.1.1.184 ethyl 2-chloro-3-oxobutanoate + NADPH + H+
-
Rhodotorula glutinis ethyl 2-chloro-3-hydroxybutanoate + NADP+
-
?
1.1.1.184 ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
Rhodotorula glutinis ethyl 4-chloro-3-hydroxybutanoate + NADP+
-
?
1.1.1.184 glutaraldehyde + NADPH low activity Rhodotorula glutinis ? + NADP+
-
?
1.1.1.184 ketopantoyl lactone + NADPH
-
Rhodotorula glutinis (R)-pantolactone + NADP+
-
?
1.1.1.184 methyl glyoxal + NADPH
-
Rhodotorula glutinis ? + NADP+
-
?
1.1.1.184 methyl pyruvate + NADPH
-
Rhodotorula glutinis methyl lactate + NADP+
-
?
1.1.1.184 additional information enzyme is highly stereospecific, broad range substrate specificity Rhodotorula glutinis ?
-
?
1.1.1.184 n-butylaldehyde + NADPH
-
Rhodotorula glutinis n-butanol + NADP+
-
?
1.1.1.184 n-hexylaldehyde + NADPH low activity Rhodotorula glutinis n-hexanol + NADP+
-
?
1.1.1.184 octyl 4-chloro-3-oxobutanoate + NADPH
-
Rhodotorula glutinis octyl 4-chloro-3-hydroxybutanoate + NADP+
-
?
1.1.1.184 oxalacetic acid + NADPH
-
Rhodotorula glutinis ? + NADP+
-
?
1.1.1.184 propionaldehyde + NADPH low activity Rhodotorula glutinis propanol + NADP+
-
?
1.1.1.184 propiophenone + NADPH
-
Rhodotorula glutinis 1-phenylpropan-1-ol + NADP+
-
?
1.1.1.184 pyridine-4-aldehyde + NADPH + H+
-
Rhodotorula glutinis pyridin-4-ylmethanol + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 monomer 1 * 30000, SDS-PAGE Rhodotorula glutinis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 carbonyl reductase
-
Rhodotorula glutinis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.184 40 50
-
Rhodotorula glutinis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.184 5 6 assay at Rhodotorula glutinis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADPH dependent on Rhodotorula glutinis