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Literature summary extracted from

  • Takasaki, K.; Shoun, H.; Nakamura, A.; Hoshino, T.; Takaya, N.
    Unusual transcription regulation of the niaD gene under anaeobic conditions supporting fungal ammonia fermentation (2004), Biosci. Biotechnol. Biochem., 68, 978-980.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.3 additional information exogenous nitrate induces the enzyme expression, can be repressed by ammonium under aerobic conditions, but not under anaerobic conditions, promotor activity study Aspergillus nidulans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.3 construction of a fusion gene comprising gene the promotor of gene niaD and the Escherichia coli beta-glucuronidase gene, i.e. GUS, for analysis of promotor activity in Aspergillus nidulans Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.3 additional information construction of null-mutants of gene niaD Aspergillus nidulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.3 additional information exogenous ammonium represses enzyme expression under oxic conditions in presence or absence of nitrate, while under anoxic conditions the enzyme is expressed even in the presence of ammonium, promotor activity study Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.3 nitrate + NADPH Aspergillus nidulans enzyme is essential for reduction of nitrate to ammonia, under oxic conditions the enzyme is involved in nitrate assimilation, under anoxic conditions the enzyme is used for dissimilatory nitrate reduction, transcription regulation mechanism via ammonium, nitrate, and O2 concentrations, overview nitrite + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.3 Aspergillus nidulans
-
 gene niaD, several strains
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.3 0.0001
-
 below, wild-type enzyme, aerobic conditions, presence of ammonium Aspergillus nidulans
1.7.1.3 0.0001
-
 below, wild-type enzyme, aerobic conditions, presence of nitrate and ammonium Aspergillus nidulans
1.7.1.3 0.012
-
 wild-type enzyme, anaerobic conditions, presence of ammonium Aspergillus nidulans
1.7.1.3 0.013
-
 wild-type enzyme, aerobic conditions, presence of nitrate Aspergillus nidulans
1.7.1.3 0.018
-
 wild-type enzyme, anaerobic conditions, presence of nitrate Aspergillus nidulans
1.7.1.3 0.019
-
 wild-type enzyme, anaerobic conditions, presence of nitrate and ammonium Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.3 nitrate + NADPH
-
 Aspergillus nidulans nitrite + NADP+
-
 ?
1.7.1.3 nitrate + NADPH enzyme is essential for reduction of nitrate to ammonia, under oxic conditions the enzyme is involved in nitrate assimilation, under anoxic conditions the enzyme is used for dissimilatory nitrate reduction, transcription regulation mechanism via ammonium, nitrate, and O2 concentrations, overview Aspergillus nidulans nitrite + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.3 nitrate reductase
-
 Aspergillus nidulans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.3 NADPH
-
 Aspergillus nidulans