Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Horikiri, S.; Aizawa, Y.; Kai, T.; Amachi, S.; Shinoyama, H.; Fujii, T.
    Electron acquisition system constructed from an NAD-independent D-lactate dehydrogenase and cytochrome c2 in Rhodopseudomonas palustris No. 7 (2004), Biosci. Biotechnol. Biochem., 68, 516-522.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.28 oxalate 0.05 mM, about 40% residual activity Rhodopseudomonas palustris
1.1.2.4 oxalate competitive Rhodopseudomonas palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.28 0.8
-
 D-lactate pH 8.0, 30°C Rhodopseudomonas palustris
1.1.2.4 0.8
-
 D-lactate pH 8.0, 30°C Rhodopseudomonas palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.28 57000
-
 x * 57000, SDS-PAGE Rhodopseudomonas palustris
1.1.1.28 235000
-
 gel filtration Rhodopseudomonas palustris
1.1.2.4 57000
-
 4 * 57000, SDS-PAGE Rhodopseudomonas palustris
1.1.2.4 235000
-
 gel filtration Rhodopseudomonas palustris

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Rhodopseudomonas palustris
-
 No. 7, grown photoanaerobically on lactate
-
1.1.2.4 Rhodopseudomonas palustris
-
 No. 7, growth photoanaerobically on lactate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.28
-
 Rhodopseudomonas palustris
1.1.2.4
-
 Rhodopseudomonas palustris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.28 13.5
-
 pH 8.0, 30°C Rhodopseudomonas palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 D-lactate + cytochrome c2ox
-
 Rhodopseudomonas palustris pyruvate + cytochrome c2red
-
 ?
1.1.1.28 D-lactate + NAD+
-
 Rhodopseudomonas palustris pyruvate + NADH
-
 ?
1.1.1.28 DL-2-hydroxybutyrate + NAD+ about 44% of activity against D-lactate Rhodopseudomonas palustris ? + NADH
-
 ?
1.1.2.4 (R)-lactate + ferricytochrome c
-
 Rhodopseudomonas palustris pyruvate + ferrocytochrome c
-
 ?
1.1.2.4 DL-2-hydroxybutyrate + ferricytochrome c 40% of the activity with (R)-lactate Rhodopseudomonas palustris 2-oxobutanoate + ferrocytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.28 tetramer x * 57000, SDS-PAGE Rhodopseudomonas palustris
1.1.2.4 tetramer 4 * 57000, SDS-PAGE Rhodopseudomonas palustris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.28 50
-
-
 Rhodopseudomonas palustris
1.1.2.4 50
-
-
 Rhodopseudomonas palustris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.28 50
-
 30 min, stable Rhodopseudomonas palustris
1.1.1.28 60
-
 above, rapid decrease of activity Rhodopseudomonas palustris
1.1.2.4 60
-
 rapid inactivation above Rhodopseudomonas palustris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.28 8.5
-
-
 Rhodopseudomonas palustris
1.1.2.4 8.5
-
-
 Rhodopseudomonas palustris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.2.4 7.5 9.5
-
 Rhodopseudomonas palustris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 FAD
-
 Rhodopseudomonas palustris
1.1.1.28 NAD+
-
 Rhodopseudomonas palustris
1.1.2.4 FAD
-
 Rhodopseudomonas palustris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.28 0.12
-
 oxalate pH 8.0, 30°C Rhodopseudomonas palustris
1.1.2.4 0.12
-
 oxalate pH 8.0, 30°C Rhodopseudomonas palustris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.28 Rhodopseudomonas palustris isoelectric focusing
-
 5
1.1.2.4 Rhodopseudomonas palustris
-
-
 5