Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kondo, T.; Kondo, E.; Maki, H.; Yasumoto, K.; Takagi, K.; Kano, K.; Ikeda, T.
    Purification and characterization of aromatic amine dehydrogenase from Alcaligenes xylosoxidans (2004), Biosci. Biotechnol. Biochem., 68, 1921-1928.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.9.2 8-hydroxyquinoline 33% inhibition at 1 mM Achromobacter xylosoxidans
1.4.9.2 beta-phenylethylamine substrate inhibition at higher concentrations Achromobacter xylosoxidans
1.4.9.2 dopamine substrate inhibition at higher concentrations Achromobacter xylosoxidans
1.4.9.2 EDTA 13% inhibition at 1 mM Achromobacter xylosoxidans
1.4.9.2 histamine weak substrate inhibition above 1 mM Achromobacter xylosoxidans
1.4.9.2 hydrazine complete inhibition at 0.1 mM Achromobacter xylosoxidans
1.4.9.2 hydroxylamine 99% inhibition at 1 mM Achromobacter xylosoxidans
1.4.9.2 iodoacetamide 28% inhibition at 1 mM Achromobacter xylosoxidans
1.4.9.2 monoiodoacetate 15% inhibition at 1 mM Achromobacter xylosoxidans
1.4.9.2 NaN3 30% inhibition at 1 mM Achromobacter xylosoxidans
1.4.9.2 o-phenanthroline 17% inhibition at 1 mM Achromobacter xylosoxidans
1.4.9.2 Semicarbazide 97% inhibition at 1 mM Achromobacter xylosoxidans
1.4.9.2 serotonin substrate inhibition at higher concentrations Achromobacter xylosoxidans
1.4.9.2 tryptamine substrate inhibition at higher concentrations Achromobacter xylosoxidans
1.4.9.2 tyramine substrate inhibition at higher concentrations Achromobacter xylosoxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.9.2 0.0018
-
 beta-phenylethylamine pH 7.5, 30°C Achromobacter xylosoxidans
1.4.9.2 0.0069
-
 tyramine pH 7.5, 30°C Achromobacter xylosoxidans
1.4.9.2 0.048
-
 phenazine methosulfate pH 7.5, 30°C Achromobacter xylosoxidans
1.4.9.2 0.171
-
 histamine pH 7.5, 30°C Achromobacter xylosoxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.9.2 15200
-
 2 * 42300, alpha-subunit, + 2 * 15200, beta-subunit, SDS-PAGE Achromobacter xylosoxidans
1.4.9.2 42300
-
 2 * 42300, alpha-subunit, + 2 * 15200, beta-subunit, SDS-PAGE Achromobacter xylosoxidans
1.4.9.2 95500
-
 gel filtration Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.9.2 beta-phenylethylamine + H2O + 2,6-dichlorophenol indophenol Achromobacter xylosoxidans
-
 phenylacetaldehyde + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 beta-phenylethylamine + H2O + 2,6-dichlorophenol indophenol Achromobacter xylosoxidans IFO13495
-
 phenylacetaldehyde + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.9.2 Achromobacter xylosoxidans
-
-
-
1.4.9.2 Achromobacter xylosoxidans IFO13495
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.9.2 61fold to homogeneity, multistep procedure Achromobacter xylosoxidans

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.9.2 ArCH2NH2 + H2O + 2 azurin = ArCHO + NH3 + 2 reduced azurin ping-pong mechanism Achromobacter xylosoxidans
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.9.2 culture condition:beta-phenylethylamine-grown cell
-
 Achromobacter xylosoxidans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.9.2 7.05
-
 purified enzyme Achromobacter xylosoxidans

Storage Stability

EC Number Storage Stability Organism
1.4.9.2 4°C, purified enzyme, phosphate buffer, pH 7.0, stable for at least 1 month Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.9.2 1,3-diaminopropane + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 1,3-diaminopropane + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans IFO13495 ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 1,6-diaminohexane + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 1,6-diaminohexane + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans IFO13495 ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 beta-phenylethylamine + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans phenylacetaldehyde + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 beta-phenylethylamine + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans IFO13495 phenylacetaldehyde + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 butylamine + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans butanal + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 butylamine + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans IFO13495 butanal + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 cadaverine + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 dopamine + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans (3,4-dihydroxyphenyl)acetaldehyde + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 hexylamine + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans hexanal + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 histamine + H2O + 2,6-dichlorophenol indophenol high activity at substrate concentration 1 mM Achromobacter xylosoxidans 1H-imidazol-4-ylacetaldehyde + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 additional information substrate specificity, overview, no activity with methylamine, dimethylamine, and trimethylamine Achromobacter xylosoxidans ?
-
 ?
1.4.9.2 additional information substrate specificity, overview, no activity with methylamine, dimethylamine, and trimethylamine Achromobacter xylosoxidans IFO13495 ?
-
 ?
1.4.9.2 pentylamine + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans pentanal + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 phenazine methosulfate + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 propylamine + H2O + 2,6-dichlorophenol indophenol low activity Achromobacter xylosoxidans propanal + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 putrescine + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans 4-aminobutanal + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 serotonin + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans 6-hydroxy-4H-indol-3-yl-acetaldehyde + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 spermidine + H2O + 2,6-dichlorophenol indophenol low activity Achromobacter xylosoxidans ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 spermine + H2O + 2,6-dichlorophenol indophenol low activity Achromobacter xylosoxidans ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 tryptamine + H2O + 2,6-dichlorophenol indophenol
-
 Achromobacter xylosoxidans 4H-indol-3-yl-acetaldehyde + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 tyramine + H2O + 2,6-dichlorophenol indophenol best substrate Achromobacter xylosoxidans 4-hydroxyphenylacetaldehyde + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.9.2 tetramer 2 * 42300, alpha-subunit, + 2 * 15200, beta-subunit, SDS-PAGE Achromobacter xylosoxidans

Synonyms

EC Number Synonyms Comment Organism
1.4.9.2 AADH
-
 Achromobacter xylosoxidans
1.4.9.2 aromatic amine dehydrogenase
-
 Achromobacter xylosoxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.9.2 70
-
-
 Achromobacter xylosoxidans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.9.2 20 80
-
 Achromobacter xylosoxidans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.9.2 70
-
 40 min, stable, no loss of activity Achromobacter xylosoxidans
1.4.9.2 80
-
 10 min, loss of 20% activity Achromobacter xylosoxidans
1.4.9.2 100
-
 10 min, inactivation Achromobacter xylosoxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.9.2 8
-
-
 Achromobacter xylosoxidans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.9.2 5 12
-
 Achromobacter xylosoxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.9.2 tryptophan tryptophylquinone
-
 Achromobacter xylosoxidans