Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Maruyama, K.; Shibayama, T.; Ichikawa, A.; Sakou, Y.; Yamada, S.; Sugisaki, H.
    Cloning and characterization of the genes encoding enzymes for the protocatechuate meta-degradation pathway of Pseudomonas ochraceae NGJ1 (2004), Biosci. Biotechnol. Biochem., 68, 1434-1441.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.312
-
 Pseudomonas straminea
3.1.1.57 expressed in Escherichia coli Pseudomonas straminea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.312 0.0061
-
 NADP+ 25°C Pseudomonas straminea
1.1.1.312 0.075
-
 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde 25°C Pseudomonas straminea
1.1.1.312 0.314
-
 NADP+ 25°C Pseudomonas straminea
3.1.1.57 0.025
-
 4-oxalomesaconate pH 6.5, 25°C Pseudomonas straminea
3.1.1.57 0.094
-
 2-Pyrone-4,6-dicarboxylate pH 8.5, 25°C Pseudomonas straminea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.312 35000
-
 2 * 35000, SDS-PAGE Pseudomonas straminea
1.1.1.312 67000
-
 gel filtration Pseudomonas straminea
3.1.1.57 30000
-
 gel filtration Pseudomonas straminea
3.1.1.57 34345
-
 1 * 34345, calculated from the deduced amino acid sequence, native mass by gel filtration Pseudomonas straminea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.57 2-pyrone-4,6-dicarboxylate + H2O Pseudomonas straminea involved in the meta-degradation pathway for protocatechuate 4-oxalomesaconate
-
 r
3.1.1.57 2-pyrone-4,6-dicarboxylate + H2O Pseudomonas straminea NGJ1 involved in the meta-degradation pathway for protocatechuate 4-oxalomesaconate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.312 Pseudomonas straminea
-
 NGJ1
-
1.1.1.312 Pseudomonas straminea NGJ1
-
 NGJ1
-
3.1.1.57 Pseudomonas straminea
-
-
-
3.1.1.57 Pseudomonas straminea NGJ1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.312 recombinant enzyme expressed in Escherichia coli Pseudomonas straminea
3.1.1.57 recombinant protein Pseudomonas straminea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.312 549
-
 25°C Pseudomonas straminea
3.1.1.57 49.6
-
 recombinant protein from Escherichia coli, 25°C Pseudomonas straminea
3.1.1.57 495
-
 purified recombinant protein from Escherichia coli, 25°C Pseudomonas straminea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.312 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+ NAD+ is less effective than NADP+ Pseudomonas straminea 4-carboxy-2-hydroxy-cis,cis-muconate + NADH
-
 ?
1.1.1.312 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+ NAD+ is less effective than NADP+ Pseudomonas straminea NGJ1 4-carboxy-2-hydroxy-cis,cis-muconate + NADH
-
 ?
1.1.1.312 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ NAD+ is less effective than NADP+ Pseudomonas straminea 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH
-
 ?
1.1.1.312 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ NAD+ is less effective than NADP+ Pseudomonas straminea NGJ1 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH
-
 ?
3.1.1.57 2-pyrone-4,6-dicarboxylate + H2O
-
 Pseudomonas straminea 4-oxalomesaconate
-
 r
3.1.1.57 2-pyrone-4,6-dicarboxylate + H2O involved in the meta-degradation pathway for protocatechuate Pseudomonas straminea 4-oxalomesaconate
-
 r
3.1.1.57 2-pyrone-4,6-dicarboxylate + H2O
-
 Pseudomonas straminea NGJ1 4-oxalomesaconate
-
 r
3.1.1.57 2-pyrone-4,6-dicarboxylate + H2O involved in the meta-degradation pathway for protocatechuate Pseudomonas straminea NGJ1 4-oxalomesaconate
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.312 dimer 2 * 35000, SDS-PAGE Pseudomonas straminea
3.1.1.57 monomer 1 * 34345, calculated from the deduced amino acid sequence, native mass by gel filtration Pseudomonas straminea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.57 6.5
-
 reverse reaction Pseudomonas straminea
3.1.1.57 8.5
-
 forward reaction Pseudomonas straminea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.312 NADP+
-
 Pseudomonas straminea