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Literature summary extracted from

  • Nanba, H.; Takaoka, Y.; Hasegawa, J.
    Purification and characterization of formate dehydrogenase from Ancylobacter aquaticus strain KNK607M, and cloning of the gene (2003), Biosci. Biotechnol. Biochem., 67, 720-728.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.9 high expression in Escherichia coli using the expression plasmid pFA002 Ancylobacter aquaticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 Cu2+ 1 mM, 37% inhibition Ancylobacter aquaticus
1.17.1.9 Hg2+ 1 mM, complete inhibition Ancylobacter aquaticus
1.17.1.9 NaN3 1 mM, complete inhibition Ancylobacter aquaticus
1.17.1.9 NEM 1 mM, 25.3% inhibition Ancylobacter aquaticus
1.17.1.9 PCMB 0.2 mM, complete inhibition Ancylobacter aquaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.057
-
 NAD+ pH 7.0, 30°C Ancylobacter aquaticus
1.17.1.9 2.4
-
 formate pH 7.0, 30°C Ancylobacter aquaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 44000
-
 2 * 44000, SDS-PAGE Ancylobacter aquaticus
1.17.1.9 100000
-
 gel filtration Ancylobacter aquaticus

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Ancylobacter aquaticus Q845T0
-
-
1.17.1.9 Ancylobacter aquaticus KNK607M Q845T0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9
-
 Ancylobacter aquaticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.9 9.47
-
-
 Ancylobacter aquaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 Ancylobacter aquaticus CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Ancylobacter aquaticus KNK607M CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NADP+ activity with NADP+ is 2.4% of the activity with NAD+ Ancylobacter aquaticus CO2 + NADPH
-
 ?
1.17.1.9 formate + NADP+ activity with NADP+ is 2.4% of the activity with NAD+ Ancylobacter aquaticus KNK607M CO2 + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 dimer 2 * 44000, SDS-PAGE Ancylobacter aquaticus

Synonyms

EC Number Synonyms Comment Organism
1.17.1.9 NAD-dependent formate dehydrogenase
-
 Ancylobacter aquaticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.9 50
-
-
 Ancylobacter aquaticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.17.1.9 35 60 35°C: about 50% of maximal activity, 60°C: about 45% of maximal activity Ancylobacter aquaticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 50
-
 pH 7.0, 10 min, stable at or lower Ancylobacter aquaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 6.3
-
-
 Ancylobacter aquaticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.1.9 5 10.5 pH 5.0: about 60% of maximal activity, pH 5.3: about 80% of maximal activity, pH 9.6: about 80% of maximal activity, pH 10.5: about 65% of maximal activity Ancylobacter aquaticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.17.1.9 5
-
 30°C, 20 h, about 50% of initial activity Ancylobacter aquaticus
1.17.1.9 7
-
 30°C, 20 h, most stable at Ancylobacter aquaticus
1.17.1.9 8
-
 30°C, 20 h, about 25% of initial activity Ancylobacter aquaticus
1.17.1.9 8.5
-
 30°C, 20 h, about 40% of initial activity Ancylobacter aquaticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 Ancylobacter aquaticus
1.17.1.9 NADP+ activity is 2.4% of the activity with NAD+ Ancylobacter aquaticus