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Literature summary extracted from

  • Kaomek, M.; Mizuno, K.; Fujimura, T.; Sriyotha, P.; Cairns, J.R.
    Cloning, expression, and characterization of an antifungal chitinase from Leucaena leucocephala de Wit (2003), Biosci. Biotechnol. Biochem., 67, 667-676.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression in Escherichia coli containing a N-terminal additional Met and a C-terminal His tag Leucaena leucocephala

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 32000
-
 x * 32000, SDS-PAGE Leucaena leucocephala

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Leucaena leucocephala
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Leucaena leucocephala

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 seed
-
 Leucaena leucocephala
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 0.057
-
 purified recombinant chitinase Leucaena leucocephala

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O colloidal, purified and swollen chitin Leucaena leucocephala ?
-
 ?
3.2.1.14 chitosan
-
 Leucaena leucocephala ?
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Leucaena leucocephala ?
-
 ?
3.2.1.14 p-nitrophenyl-beta-D-N,N',N''-triacetylchitotriose + H2O
-
 Leucaena leucocephala N,N',N''-triacetylchitotriose + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 32000, SDS-PAGE Leucaena leucocephala

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 55
-
-
 Leucaena leucocephala

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 4.5
-
-
 Leucaena leucocephala

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Leucaena leucocephala isoelectric focusing
-
 7.5