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Literature summary extracted from

  • Sripuan, T.; Aoki, K.; Yamamoto, K.; Tongkao, D.; Kumagai, H.
    Purification and characterization of thermostable alpha-galactosidase from Ganoderma lucidum (2003), Biosci. Biotechnol. Biochem., 67, 1485-1491.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ 1 mM, 68% inhibition Ganoderma lucidum
3.2.1.22 D-galactose 10 mM, complete inhibition Ganoderma lucidum
3.2.1.22 D-xylose 10 mM, 12% inhibition Ganoderma lucidum
3.2.1.22 Hg2+ 1 mM, 91% inhibition Ganoderma lucidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.4
-
p-nitrophenyl alpha-D-galactopyranoside pH 6.0, 40°C Ganoderma lucidum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 Cu2+ 1 mM, 1.2fold stimulation Ganoderma lucidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 56000
-
4 * 56000, SDS-PAGE Ganoderma lucidum
3.2.1.22 249000
-
gel filtration Ganoderma lucidum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.22 melibiose + H2O Ganoderma lucidum 42.1% of the activity with p-nitrophenyl alpha-D-galactopyranoside alpha-D-galactose + D-glucose
-
?
3.2.1.22 o-nitrophenyl alpha-D-galactopyranoside + H2O Ganoderma lucidum 27.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside o-nitrophenol + D-galactose
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + D-glucose Ganoderma lucidum transglycosylation ?
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O Ganoderma lucidum
-
p-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 raffinose + H2O Ganoderma lucidum 52.4% of the activity with p-nitrophenyl alpha-D-galactopyranoside sucrose + alpha-D-galactose
-
?
3.2.1.22 stachyose + 2 H2O Ganoderma lucidum 69.7% of the activity with p-nitrophenyl alpha-D-galactopyranoside 2 alpha-D-galactose + sucrose
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Ganoderma lucidum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
Ganoderma lucidum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.22 fruit body fresh Ganoderma lucidum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 23.2
-
-
Ganoderma lucidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 melibiose + H2O 42.1% of the activity with p-nitrophenyl alpha-D-galactopyranoside Ganoderma lucidum alpha-D-galactose + D-glucose
-
?
3.2.1.22 o-nitrophenyl alpha-D-galactopyranoside + H2O 27.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside Ganoderma lucidum o-nitrophenol + D-galactose
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + D-glucose transglycosylation Ganoderma lucidum ?
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Ganoderma lucidum p-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 raffinose + H2O 52.4% of the activity with p-nitrophenyl alpha-D-galactopyranoside Ganoderma lucidum sucrose + alpha-D-galactose
-
?
3.2.1.22 stachyose + 2 H2O 69.7% of the activity with p-nitrophenyl alpha-D-galactopyranoside Ganoderma lucidum 2 alpha-D-galactose + sucrose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 tetramer 4 * 56000, SDS-PAGE Ganoderma lucidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 70
-
-
Ganoderma lucidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 70
-
30 min, stable Ganoderma lucidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 6
-
-
Ganoderma lucidum