EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.57 | ipuA gene, expression in Pichia pastoris GS115 | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.57 | Aspergillus niger | - |
strain ATCC 9642 | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.57 | glycoprotein | recombinant IPU expressed in Pichia pastoris GS115 has a higher carbohydrate content, 41%, than native IPU, 12-15%, or recombinant IPU expressed in Aspergillus oryzae M-2-3, 34% | Aspergillus niger |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.57 | recombinant IPU expressed in Pichia pastoris | Aspergillus niger |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.57 | 25.2 | - |
pH 3.5, 40°C, recombinant IPU expressed in Pichia pastoris | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.57 | 4(2)-alpha-isomaltosylisomaltose + H2O | cleaves the alpha-1,4-glycosidic linkage of the panose motif | Aspergillus niger | ? | - |
? | |
3.2.1.57 | 4(3)-alpha-panosylpanose + H2O | cleaves the alpha-1,4-glycosidic linkage of the panose motif | Aspergillus niger | ? | - |
? | |
3.2.1.57 | 6(2)-alpha-isomaltosylmaltose + H2O | isomaltotriosylglucose, cleaves the alpha-1,4-glycosidic linkage of the panose motif | Aspergillus niger | ? | - |
? | |
3.2.1.57 | 6(2)-alpha-maltosylmaltose + H2O | cleaves the alpha-1,4-glycosidic linkage of the panose motif | Aspergillus niger | ? | - |
? | |
3.2.1.57 | 6(3)-alpha-glucosylmaltotriose + H2O | cleaves the alpha-1,4-glycosidic linkage of the panose motif | Aspergillus niger | ? | - |
? | |
3.2.1.57 | additional information | recombinant IPU hydrolyzes various substrates containing the structure of panose indicating a strict subsite recognition of the panose motif, cleaves the alpha-1,4-glycosidic linkage | Aspergillus niger | ? | - |
? | |
3.2.1.57 | panose + H2O | cleaves the alpha-1,4-glycosidic linkage | Aspergillus niger | isomaltose + glucose | - |
? | |
3.2.1.57 | pullulan + H2O | cleaves the alpha-1,4-glycosidic linkage | Aspergillus niger | isopanose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.57 | IPU | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.57 | 40 | - |
assay at | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.57 | 3.5 | - |
assay at | Aspergillus niger |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.57 | 2.4 | 7 | recombinant IPU expressed in Pichia pastoris, at 4°C, retains pullulan-hydrolyzing activity for 12 hours | Aspergillus niger |