Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yoon, J.J.; Hattori, T.; Shimada, M.
    Purification and characterization of NADP-linked isocitrate dehydrogenase from the copper-tolerant wood-rotting basidiomycete Fomitopsis palustris (2003), Biosci. Biotechnol. Biochem., 67, 114-120.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.42 Mg2+ stabilizes the enzyme during purification Fomitopsis palustris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 2-oxoglutarate 92% inhibition at 5 mM Fomitopsis palustris
1.1.1.42 ADP 15% inhibition at 2 mM Fomitopsis palustris
1.1.1.42 ATP 10% inhibition at 2 mM Fomitopsis palustris
1.1.1.42 Ca2+ in presence of 5 mM Mg2+ 6% of maximal activity is remaining Fomitopsis palustris
1.1.1.42 Cd2+ complete inhibition at 5 mM in presence of 5 mM Mg2+ Fomitopsis palustris
1.1.1.42 cis-aconitate 35% inhibition at 5 mM Fomitopsis palustris
1.1.1.42 citrate 30% inhibition at 1 mM and 5 mM Fomitopsis palustris
1.1.1.42 Co2+ 92% inhibition in presence of 5 mM Mg2+ Fomitopsis palustris
1.1.1.42 Cu2+ enzyme is completely inhibited at 5 mM in presence of 5 mM Mg2+, organism is a copper-tolerant basidiomycete Fomitopsis palustris
1.1.1.42 glyoxylate 18% inhibition at 5 mM, mixed type inhibition together with oxaloacetate, completely at 5 mM each Fomitopsis palustris
1.1.1.42 Itaconate 18% inhibition at 5 mM Fomitopsis palustris
1.1.1.42 additional information AMP is a no inhibitor, no inhibition by glutamate, pyruvate and succinate Fomitopsis palustris
1.1.1.42 oxalate 18% inhibition at 5 mM Fomitopsis palustris
1.1.1.42 oxaloacetate 41% inhibition at 5 mM, mixed type inhibition together with glyoxylate, completely at 5 mM each Fomitopsis palustris
1.1.1.42 Zn2+ complete inhibition at 5 mM in presence of 5 mM Mg2+ Fomitopsis palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.0029
-
 NADP+ pH 9.0, 30°C Fomitopsis palustris
1.1.1.42 0.0127
-
 isocitrate pH 9.0, 30°C Fomitopsis palustris
1.1.1.42 0.0239
-
 Mg2+ pH 9.0, 30°C Fomitopsis palustris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 cytosol
-
 Fomitopsis palustris 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Co2+ can substitute for Mg2+ by 25% Fomitopsis palustris
1.1.1.42 Mg2+ required for activity Fomitopsis palustris
1.1.1.42 Mn2+ can substitute for Mg2+ by 90% Fomitopsis palustris
1.1.1.42 additional information K+ cannot substitute for Mg2+ Fomitopsis palustris
1.1.1.42 Na+ can substitute for Mg2+ by 20% Fomitopsis palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 55000
-
 2 * 55000, SDS-PAGE Fomitopsis palustris
1.1.1.42 115000
-
 gel filtration Fomitopsis palustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Fomitopsis palustris key enzyme of the tricarboxylic acid cycle 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Fomitopsis palustris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 672fold to homogeneity Fomitopsis palustris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.42 mycelium
-
 Fomitopsis palustris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.42 75
-
 purified enzyme Fomitopsis palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 DL-isocitrate + NADP+
-
 Fomitopsis palustris 2-oxoglutarate + CO2 + NADPH
-
 ?
1.1.1.42 isocitrate + NADP+ key enzyme of the tricarboxylic acid cycle Fomitopsis palustris 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.42 dimer 2 * 55000, SDS-PAGE Fomitopsis palustris

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 NADP-dependent isocitrate dehydrogenase
-
 Fomitopsis palustris
1.1.1.42 NADP-IDH
-
 Fomitopsis palustris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 30
-
-
 Fomitopsis palustris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 9
-
-
 Fomitopsis palustris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Fomitopsis palustris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.42 0.127
-
 2-oxoglutarate pH 9.0, 30°C Fomitopsis palustris