BRENDA - Enzyme Database

Purification and characterization of NADP-linked isocitrate dehydrogenase from the copper-tolerant wood-rotting basidiomycete Fomitopsis palustris

Yoon, J.J.; Hattori, T.; Shimada, M.; Biosci. Biotechnol. Biochem. 67, 114-120 (2003)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.1.1.42
Mg2+ stabilizes the enzyme during purification
Fomitopsis palustris
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
92% inhibition at 5 mM
Fomitopsis palustris
1.1.1.42
ADP
15% inhibition at 2 mM
Fomitopsis palustris
1.1.1.42
ATP
10% inhibition at 2 mM
Fomitopsis palustris
1.1.1.42
Ca2+
in presence of 5 mM Mg2+ 6% of maximal activity is remaining
Fomitopsis palustris
1.1.1.42
Cd2+
complete inhibition at 5 mM in presence of 5 mM Mg2+
Fomitopsis palustris
1.1.1.42
cis-aconitate
35% inhibition at 5 mM
Fomitopsis palustris
1.1.1.42
citrate
30% inhibition at 1 mM and 5 mM
Fomitopsis palustris
1.1.1.42
Co2+
92% inhibition in presence of 5 mM Mg2+
Fomitopsis palustris
1.1.1.42
Cu2+
enzyme is completely inhibited at 5 mM in presence of 5 mM Mg2+, organism is a copper-tolerant basidiomycete
Fomitopsis palustris
1.1.1.42
glyoxylate
18% inhibition at 5 mM, mixed type inhibition together with oxaloacetate, completely at 5 mM each
Fomitopsis palustris
1.1.1.42
Itaconate
18% inhibition at 5 mM
Fomitopsis palustris
1.1.1.42
additional information
AMP is a no inhibitor, no inhibition by glutamate, pyruvate and succinate
Fomitopsis palustris
1.1.1.42
oxalate
18% inhibition at 5 mM
Fomitopsis palustris
1.1.1.42
oxaloacetate
41% inhibition at 5 mM, mixed type inhibition together with glyoxylate, completely at 5 mM each
Fomitopsis palustris
1.1.1.42
Zn2+
complete inhibition at 5 mM in presence of 5 mM Mg2+
Fomitopsis palustris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0029
-
NADP+
pH 9.0, 30C
Fomitopsis palustris
1.1.1.42
0.0127
-
isocitrate
pH 9.0, 30C
Fomitopsis palustris
1.1.1.42
0.0239
-
Mg2+
pH 9.0, 30C
Fomitopsis palustris
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Fomitopsis palustris
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Co2+
can substitute for Mg2+ by 25%
Fomitopsis palustris
1.1.1.42
Mg2+
required for activity
Fomitopsis palustris
1.1.1.42
Mn2+
can substitute for Mg2+ by 90%
Fomitopsis palustris
1.1.1.42
additional information
K+ cannot substitute for Mg2+
Fomitopsis palustris
1.1.1.42
Na+
can substitute for Mg2+ by 20%
Fomitopsis palustris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
55000
-
2 * 55000, SDS-PAGE
Fomitopsis palustris
1.1.1.42
115000
-
gel filtration
Fomitopsis palustris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Fomitopsis palustris
key enzyme of the tricarboxylic acid cycle
2-oxoglutarate + CO2 + NADPH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Fomitopsis palustris
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
672fold to homogeneity
Fomitopsis palustris
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
mycelium
-
Fomitopsis palustris
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.42
75
-
purified enzyme
Fomitopsis palustris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
DL-isocitrate + NADP+
-
655035
Fomitopsis palustris
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
key enzyme of the tricarboxylic acid cycle
655035
Fomitopsis palustris
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
2 * 55000, SDS-PAGE
Fomitopsis palustris
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
30
-
-
Fomitopsis palustris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
9
-
-
Fomitopsis palustris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Fomitopsis palustris
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.42
0.127
-
2-oxoglutarate
pH 9.0, 30C
Fomitopsis palustris
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Fomitopsis palustris
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.42
Mg2+ stabilizes the enzyme during purification
Fomitopsis palustris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
92% inhibition at 5 mM
Fomitopsis palustris
1.1.1.42
ADP
15% inhibition at 2 mM
Fomitopsis palustris
1.1.1.42
ATP
10% inhibition at 2 mM
Fomitopsis palustris
1.1.1.42
Ca2+
in presence of 5 mM Mg2+ 6% of maximal activity is remaining
Fomitopsis palustris
1.1.1.42
Cd2+
complete inhibition at 5 mM in presence of 5 mM Mg2+
Fomitopsis palustris
1.1.1.42
cis-aconitate
35% inhibition at 5 mM
Fomitopsis palustris
1.1.1.42
citrate
30% inhibition at 1 mM and 5 mM
Fomitopsis palustris
1.1.1.42
Co2+
92% inhibition in presence of 5 mM Mg2+
Fomitopsis palustris
1.1.1.42
Cu2+
enzyme is completely inhibited at 5 mM in presence of 5 mM Mg2+, organism is a copper-tolerant basidiomycete
Fomitopsis palustris
1.1.1.42
glyoxylate
18% inhibition at 5 mM, mixed type inhibition together with oxaloacetate, completely at 5 mM each
Fomitopsis palustris
1.1.1.42
Itaconate
18% inhibition at 5 mM
Fomitopsis palustris
1.1.1.42
additional information
AMP is a no inhibitor, no inhibition by glutamate, pyruvate and succinate
Fomitopsis palustris
1.1.1.42
oxalate
18% inhibition at 5 mM
Fomitopsis palustris
1.1.1.42
oxaloacetate
41% inhibition at 5 mM, mixed type inhibition together with glyoxylate, completely at 5 mM each
Fomitopsis palustris
1.1.1.42
Zn2+
complete inhibition at 5 mM in presence of 5 mM Mg2+
Fomitopsis palustris
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.42
0.127
-
2-oxoglutarate
pH 9.0, 30C
Fomitopsis palustris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0029
-
NADP+
pH 9.0, 30C
Fomitopsis palustris
1.1.1.42
0.0127
-
isocitrate
pH 9.0, 30C
Fomitopsis palustris
1.1.1.42
0.0239
-
Mg2+
pH 9.0, 30C
Fomitopsis palustris
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Fomitopsis palustris
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Co2+
can substitute for Mg2+ by 25%
Fomitopsis palustris
1.1.1.42
Mg2+
required for activity
Fomitopsis palustris
1.1.1.42
Mn2+
can substitute for Mg2+ by 90%
Fomitopsis palustris
1.1.1.42
additional information
K+ cannot substitute for Mg2+
Fomitopsis palustris
1.1.1.42
Na+
can substitute for Mg2+ by 20%
Fomitopsis palustris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
55000
-
2 * 55000, SDS-PAGE
Fomitopsis palustris
1.1.1.42
115000
-
gel filtration
Fomitopsis palustris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Fomitopsis palustris
key enzyme of the tricarboxylic acid cycle
2-oxoglutarate + CO2 + NADPH
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
672fold to homogeneity
Fomitopsis palustris
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
mycelium
-
Fomitopsis palustris
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.42
75
-
purified enzyme
Fomitopsis palustris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
DL-isocitrate + NADP+
-
655035
Fomitopsis palustris
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
key enzyme of the tricarboxylic acid cycle
655035
Fomitopsis palustris
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
2 * 55000, SDS-PAGE
Fomitopsis palustris
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.42
30
-
-
Fomitopsis palustris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
9
-
-
Fomitopsis palustris