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Literature summary extracted from
- Production and characterization of recombinant Phanerochaete chrysosporium beta-glucosidase in the methylotrophic yeast Pichia pastoris (2003), Biosci. Biotechnol. Biochem., 67, 1-7.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | expression in Pichia pastoris | Phanerodontia chrysosporium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | delta-gluconolactone | - |
Phanerodontia chrysosporium |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.12 | - |
p-nitrophenyl-beta-D-glucose | wild-type BGL | Phanerodontia chrysosporium | |
| 3.2.1.21 | 0.2 | - |
p-nitrophenyl-beta-D-glucose | recombinant BGL | Phanerodontia chrysosporium | |
| 3.2.1.21 | 4.06 | - |
cellobiose | wild-type BGL | Phanerodontia chrysosporium | |
| 3.2.1.21 | 5.05 | - |
cellobiose | recombinant BGL | Phanerodontia chrysosporium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 116000 | - |
x * 116000, SDS-PAGE, wild-type BGL | Phanerodontia chrysosporium |
| 3.2.1.21 | 133000 | - |
x * 133000, SDS-PAGE, recombinant BGL | Phanerodontia chrysosporium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Phanerodontia chrysosporium | Q8TGC6 | white-rot fungus | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | recombinant and wild-type beta-glucosidase | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | cellobiose + H2O | - |
Phanerodontia chrysosporium | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Phanerodontia chrysosporium | p-nitrophenol + beta-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | ? | x * 116000, SDS-PAGE, wild-type BGL | Phanerodontia chrysosporium |
| 3.2.1.21 | ? | x * 133000, SDS-PAGE, recombinant BGL | Phanerodontia chrysosporium |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | Bgl | - |
Phanerodontia chrysosporium |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 27.5 | - |
cellobiose | wild-type BGL | Phanerodontia chrysosporium | |
| 3.2.1.21 | 29.8 | - |
cellobiose | recombinant BGL | Phanerodontia chrysosporium | |
| 3.2.1.21 | 96.2 | - |
p-nitrophenyl-beta-D-glucose | wild-type BGL | Phanerodontia chrysosporium | |
| 3.2.1.21 | 103.1 | - |
p-nitrophenyl-beta-D-glucose | recombinant BGL | Phanerodontia chrysosporium | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 2.2 | - |
delta-gluconolactone | hydrolysis of p-nitrophenyl-beta-D-glucopyranoside | Phanerodontia chrysosporium | |
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Release 2023.1 (January 2023)
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