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Literature summary extracted from

  • Shinjoh, M.; Tomiyama, N.; Miyazaki, T.; Hoshino, T.
    Main polyol dehydrogenase of Gluconobacter suboxydans IFO 3255, membrane-bound D-sorbitol dehydrogenase, that needs product of upstream gene, sldB, for activity (2002), Biosci. Biotechnol. Biochem., 66, 2314-2322.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.14 additional information enzyme needs product, i.e. SldB, of upstream gene sldB, encoding a hydrophobic polypeptide, for activity, the polypeptide might be a chaperone-like component that signals processing and folding of the enzyme to give the active enzyme form Gluconobacter oxydans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.14 expression of wild-type and mutant enzymes in Escherichia coli Gluconobacter oxydans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.14 additional information construction of disruption mutants of genes sldA or sldB, the mutants are inactive, the sldB mutation leads to a higher enzyme expression and accumulation of unprocessed SldA Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.14 membrane bound Gluconobacter oxydans 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.14 D-sorbitol + NAD+ Gluconobacter oxydans enzyme is the main L-sorbose producing activity in the cell L-sorbose + NADH + H+
-
?
1.1.1.14 D-sorbitol + NAD+ Gluconobacter oxydans IFO 3255 enzyme is the main L-sorbose producing activity in the cell L-sorbose + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Gluconobacter oxydans
-
gene sldA
-
1.1.1.14 Gluconobacter oxydans IFO 3255
-
gene sldA
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.14 additional information
-
activity at growth in media with different pH values Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-sorbitol + NAD+
-
Gluconobacter oxydans L-sorbose + NADH + H+
-
?
1.1.1.14 D-sorbitol + NAD+ enzyme is the main L-sorbose producing activity in the cell Gluconobacter oxydans L-sorbose + NADH + H+
-
?
1.1.1.14 D-sorbitol + NAD+
-
Gluconobacter oxydans IFO 3255 L-sorbose + NADH + H+
-
?
1.1.1.14 D-sorbitol + NAD+ enzyme is the main L-sorbose producing activity in the cell Gluconobacter oxydans IFO 3255 L-sorbose + NADH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.14 D-sorbitol dehydrogenase
-
Gluconobacter oxydans
1.1.1.14 polyol dehydrogenase
-
Gluconobacter oxydans
1.1.1.14 SldA
-
Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.14 25
-
assay at Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 6
-
assay at Gluconobacter oxydans