EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.14 | additional information | enzyme needs product, i.e. SldB, of upstream gene sldB, encoding a hydrophobic polypeptide, for activity, the polypeptide might be a chaperone-like component that signals processing and folding of the enzyme to give the active enzyme form | Gluconobacter oxydans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.14 | expression of wild-type and mutant enzymes in Escherichia coli | Gluconobacter oxydans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.14 | additional information | construction of disruption mutants of genes sldA or sldB, the mutants are inactive, the sldB mutation leads to a higher enzyme expression and accumulation of unprocessed SldA | Gluconobacter oxydans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.14 | membrane | bound | Gluconobacter oxydans | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.14 | D-sorbitol + NAD+ | Gluconobacter oxydans | enzyme is the main L-sorbose producing activity in the cell | L-sorbose + NADH + H+ | - |
? | |
1.1.1.14 | D-sorbitol + NAD+ | Gluconobacter oxydans IFO 3255 | enzyme is the main L-sorbose producing activity in the cell | L-sorbose + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.14 | Gluconobacter oxydans | - |
gene sldA | - |
1.1.1.14 | Gluconobacter oxydans IFO 3255 | - |
gene sldA | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.14 | additional information | - |
activity at growth in media with different pH values | Gluconobacter oxydans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.14 | D-sorbitol + NAD+ | - |
Gluconobacter oxydans | L-sorbose + NADH + H+ | - |
? | |
1.1.1.14 | D-sorbitol + NAD+ | enzyme is the main L-sorbose producing activity in the cell | Gluconobacter oxydans | L-sorbose + NADH + H+ | - |
? | |
1.1.1.14 | D-sorbitol + NAD+ | - |
Gluconobacter oxydans IFO 3255 | L-sorbose + NADH + H+ | - |
? | |
1.1.1.14 | D-sorbitol + NAD+ | enzyme is the main L-sorbose producing activity in the cell | Gluconobacter oxydans IFO 3255 | L-sorbose + NADH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.14 | D-sorbitol dehydrogenase | - |
Gluconobacter oxydans |
1.1.1.14 | polyol dehydrogenase | - |
Gluconobacter oxydans |
1.1.1.14 | SldA | - |
Gluconobacter oxydans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.14 | 25 | - |
assay at | Gluconobacter oxydans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.14 | 6 | - |
assay at | Gluconobacter oxydans |