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Literature summary extracted from

  • Moriyama, S.; Akimoto, H.; Suetsugu, N.; Kawasaki, S.; Nakamura, T.; Ohta, K.
    Purification and properties of an extracellular exoinulinase from Penicillium sp. strain TN-88 and sequence analysis of the encoding gene (2002), Biosci. Biotechnol. Biochem., 66, 1887-1896.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.80 additional information fuel production Penicillium sp.
3.2.1.80 nutrition production of fructose syrup Penicillium sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 in Escherichia coli Penicillium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 Ag+ 1.0 mM, pH 5.0, at 30ºC, 100% inhibition Penicillium sp.
3.2.1.80 Cu2+ 1.0 mM, pH 5.0, at 30ºC, 26% inhibition Penicillium sp.
3.2.1.80 EDTA 1.0 mM, pH 5.0, at 30ºC, 12% inhibition Penicillium sp.
3.2.1.80 Fe3+ 1.0 mM, pH 5.0, at 30ºC, 62% inhibition Penicillium sp.
3.2.1.80 Hg2+ 1.0 mM, pH 5.0, at 30ºC, 100% inhibition Penicillium sp.
3.2.1.80 N-bromosuccinimide 1.0 mM, pH 5.0, at 30ºC, 100% inhibition Penicillium sp.
3.2.1.80 p-chloromercuribenzoate 1.0 mM, pH 5.0, at 30ºC, 65% inhibition Penicillium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.80 0.0926
-
 inulin at 40ºC, pH 5.0 Penicillium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.80 extracellular
-
 Penicillium sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.80 Co2+ 1.0 mM, pH 5.0, at 30ºC, 117% of the relative activity without any added compound Penicillium sp.
3.2.1.80 Mn2+ 1.0 mM, pH 5.0, at 30ºC, 127% of the relative activity without any added compound Penicillium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.80 74520
-
 calculation from sequence of amino acid Penicillium sp.
3.2.1.80 81000
-
 SDS-PAGE Penicillium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.80 inulin + 5 H2O Penicillium sp.
-
 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 inulin + 5 H2O Penicillium sp. TN-88
-
 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Penicillium sp. Q8J0G1
-
-
3.2.1.80 Penicillium sp. TN-88 Q8J0G1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.80 no modification
-
 Penicillium sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 by ultrafiltration, DEAE-cellulofine column, Q-Sepharose column and Superdex column, 329fold Penicillium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.80 culture filtrate
-
 Penicillium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.80 93.8
-
 sucrose, at 40ºC, pH 5.0 Penicillium sp.
3.2.1.80 743
-
 inulin, at 40ºC, pH 5.0 Penicillium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 inulin + 5 H2O
-
 Penicillium sp. 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 inulin + 5 H2O no activity with bacterial levan Penicillium sp. 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 inulin + 5 H2O
-
 Penicillium sp. TN-88 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 inulin + 5 H2O no activity with bacterial levan Penicillium sp. TN-88 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 raffinose + H2O 18% of the activity toward inulin Penicillium sp. beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?
3.2.1.80 raffinose + H2O 18% of the activity toward inulin Penicillium sp. TN-88 beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?
3.2.1.80 sucrose + H2O
-
 Penicillium sp. beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.80 sucrose + H2O
-
 Penicillium sp. TN-88 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 exoinulinase
-
 Penicillium sp.
3.2.1.80 P-I
-
 Penicillium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.80 55
-
 pH 5.0 Penicillium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.80 50 70 pH 5.0, enzyme remains stable up to 50ºC, but it loses activity at 70ºC Penicillium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 4
-
 at 40ºC Penicillium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.80 5 7 at 30ºC Penicillium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.80 Penicillium sp. analytical isoelectric focusing
-
 4.9
3.2.1.80 Penicillium sp. deduction from sequence of amino acid
-
 4.9