Literature summary extracted from

Watanabe, N.; Takayama, S.; Yoshida, S.; Isogai, A.; Che, F.S.
Resistance to protoporphyrinogen oxidase-inhibiting compound S23142 from overproduction of mitochondrial protoporphyrinogen oxidase by gene amplification in photomixotrophic tobacco cells (2002), Biosci. Biotechnol. Biochem., 66, 1799-1805.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.3.3.4	overproduction of mitochondrial isozyme by gene amplification in photomixotrophic cells	Nicotiana tabacum

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.3.3.4	5-[2-chloro-4-(trifluoromethyl)phenoxyl]-2-nitrobenzoic acid	transgenic YZI-1S cells show a 150fold greater resistance to the inhibitor compared to wild-type cells, inhibition of mitochondrial and chloroplastic isozymes	Nicotiana tabacum

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.3.3.4	chloroplast	plastidic isozyme	Nicotiana tabacum	9507	-
1.3.3.4	membrane	both isozymes	Nicotiana tabacum	16020	-
1.3.3.4	mitochondrion	mitochondrial isozyme	Nicotiana tabacum	5739	-
1.3.3.4	additional information	activity in chloroplast of wild-type and transgenic cells is similar for both isozymes, while the transgenic mitochondria show 5.3fold higher activity than those from the wild-type	Nicotiana tabacum	-	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.3.3.4	protoporphyrinogen-IX + 3 O2	Nicotiana tabacum	-	protoporphyrin-IX + 3 H2O2	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.3.3.4	Nicotiana tabacum	-	cv. Samsun NN, 2 isozymes in chloroplasts and mitochondria	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.3.3.4	both isozymes partially from cell culture of wild-type and transgenic cells, by chloroplast and mitochondria fractionation, detergent solubilization, and ultracentrifugation	Nicotiana tabacum

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.3.3.4	cell culture	photomixotrophic cells	Nicotiana tabacum	-
1.3.3.4	YZI-1S cell	transgenic cell with 150fold greater resistance to inhibitor S23142	Nicotiana tabacum	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.3.3.4	additional information	-	activity in chloroplast of wild-type and transgenic cells is similar for both isozymes, while the transgenic mitochondria show 5.3fold higher activity than those from the wild-type	Nicotiana tabacum

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.3.3.4	protoporphyrinogen-IX + 3 O2	-	Nicotiana tabacum	protoporphyrin-IX + 3 H2O2	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.3.3.4	protox	-	Nicotiana tabacum

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.3.3.4	7.5	-	assay at	Nicotiana tabacum

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.3.3.4	FAD	-	Nicotiana tabacum

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Release 2023.1 (January 2023)