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Literature summary extracted from

  • Kobayashi, T.; Higaki, N.; Yajima, N.; Suzumatsu, A.; Hagihara, H.; Kawai, S.; Ito, S.
    Purification and properties of a galacturonic acid-releasing exopolygalacturonase from a strain of Bacillus (2001), Biosci. Biotechnol. Biochem., 65, 842-847.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 Cu2+ 0.5 mM, 40-50% inhibition Bacillus sp. (in: Bacteria)
3.2.1.67 EDTA 10 mM, 10% inhibition Bacillus sp. (in: Bacteria)
3.2.1.67 N-bromosuccinimide 0.5 mM, 67% inhibition Bacillus sp. (in: Bacteria)
3.2.1.67 Ni2+ 0.5 mM, 40-50% inhibition Bacillus sp. (in: Bacteria)
3.2.1.67 p-chloromercuribenzoate 0.5 mM, 29% inhibition Bacillus sp. (in: Bacteria)
3.2.1.67 Pd2+ 0.5 mM, 40-50% inhibition Bacillus sp. (in: Bacteria)
3.2.1.67 Zn2+ 0.5 mM, 40-50% inhibition Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.67 additional information no requirement for metal ions Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Bacillus sp. (in: Bacteria)
-
 KSM-P443
-
3.2.1.67 Bacillus sp. (in: Bacteria) KSM-P443
-
 KSM-P443
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.67 54
-
 pH 6.8, 30°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 (1,4-alpha-D-galacturonide)n + H2O
-
 Bacillus sp. (in: Bacteria) D-galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)n + H2O
-
 Bacillus sp. (in: Bacteria) KSM-P443 D-galacturonic acid
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 50
-
 stable up to Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 7
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 7 11.5
-
 Bacillus sp. (in: Bacteria)