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Literature summary extracted from

  • Yoshida, M.; Ohira, T.; Igarashi, K.; Nagasawa, H.; Aida, K.; Hallberg, B.M.; Divne, C.; Nishino, T.; samejima, M.
    Production and characterization of recombinant Phanerochaete chrysosporium cellobiose dehydrogenase in the methylotrophic yeast Pichia pastoris (2001), Biosci. Biotechnol. Biochem., 65, 2050-2057.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.99.18 biotechnology the Pichia expression system is well suited for high-level production of recombinant enzyme Phanerodontia chrysosporium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.18 expression in Pichia pastoris, enzyme retains catalytic and cellulose-binding properties of the wild-type enzyme Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.00126
-
 cytochrome c pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium
1.1.99.18 0.00144
-
 cytochrome c pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium
1.1.99.18 0.0432
-
 cellobiose pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium
1.1.99.18 0.0581
-
 cellobiose pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium
1.1.99.18 0.327
-
 ubiquinone pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium
1.1.99.18 0.333
-
 ubiquinone pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 97000
-
 x * 97000, wild-type enzyme, SDS-PAGE Phanerodontia chrysosporium
1.1.99.18 98000
-
 x * 98000, recombinant enzyme, SDS-PAGE Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Phanerodontia chrysosporium
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.18 glycoprotein MW after deglycosylation is 92000 for the recombinant enzyme and 90000 for wild-type enzyme Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + cytochrome c
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 cellobiose + ubiquinone
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced ubiquinone
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.18 ? x * 97000, wild-type enzyme, SDS-PAGE Phanerodontia chrysosporium
1.1.99.18 ? x * 98000, recombinant enzyme, SDS-PAGE Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 35.4
-
 cytochrome c pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium
1.1.99.18 37.3
-
 cytochrome c pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium
1.1.99.18 38.6
-
 cellobiose pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium
1.1.99.18 40
-
 cellobiose pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium
1.1.99.18 43.5
-
 ubiquinone pH 4.0, 30°C, wild-type enzyme Phanerodontia chrysosporium
1.1.99.18 44.2
-
 ubiquinone pH 4.0, 30°C, recombinant enzyme Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 flavin recombinant enzyme contains 0.96 mol of flavin per mol of enzyme, wild-type enzyme contains 0.97 mol of flavin per mol of enzyme Phanerodontia chrysosporium
1.1.99.18 heme contains one heme cofactor per protein molecule Phanerodontia chrysosporium