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Literature summary extracted from

  • Park, S.Y.; Bae, E.A.; Sung, J.H.; Lee, S.K.; Kim, D.H.
    Purification and characterization of ginsenoside Rb1-metabolizing beta-glucosidase from Fusobacterium K-60, a human intestinal anaerobic bacterium (2001), Biosci. Biotechnol. Biochem., 65, 1163-1169.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 2,3-Butanediol more than2 mM, 50% inhibition Fusobacterium sp.
3.2.1.21 Ba2+ 63% inhibition Fusobacterium sp.
3.2.1.21 carbodiimide more than 2 mM, 50% inhibition Fusobacterium sp.
3.2.1.21 D-glucose 6.8 mM, 50% inhibition Fusobacterium sp.
3.2.1.21 D-mannose 5.1 mM, 50% inhibition Fusobacterium sp.
3.2.1.21 dithiothreitol more than 2 mM, 50% inhibition Fusobacterium sp.
3.2.1.21 Fe2+ 57% inhibition Fusobacterium sp.
3.2.1.21 iodoacetic acid more than 2 mM, 50% inhibition Fusobacterium sp.
3.2.1.21 Li+ 40% inhibition Fusobacterium sp.
3.2.1.21 N-ethylmaleinimide more than 2 mM, 50% inhibition Fusobacterium sp.
3.2.1.21 p-chloromercuryphenylsulfonic acid 0.0015 mM, 50% inhibition Fusobacterium sp.
3.2.1.21 paraoxon 1.7 mM, 50% inhibition Fusobacterium sp.
3.2.1.21 saccharic acid 1,4-lacotone 3.7 mM, 50% inhibition Fusobacterium sp.
3.2.1.21 tosyl-L-lysine chlormethyl ketone more than 2 mM, 50% inhibition Fusobacterium sp.
3.2.1.21 Tris 6.7 mM, 50% inhibition Fusobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 1.5
-
 p-nitrophenyl-beta-D-glucopyranoside 37°C, pH 6.0 Fusobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 80000
-
 4 * 80000, SDS-PAGE Fusobacterium sp.
3.2.1.21 320000
-
 gel filtration Fusobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Fusobacterium sp.
-
 K-60, human intestinal bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 ammonium sulfate, Butyl-Toyopearl, hydroxyapatite, Q-Sepharose, Sephacryl S-300 Fusobacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 1.52
-
-
 Fusobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 amygdalin + 2 H2O 22% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. mandelonitrile + 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O 3.6% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. 2 beta-D-glucose
-
 ?
3.2.1.21 esculin + H2O 100% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. esculetin + beta-D-glucose
-
 ?
3.2.1.21 gentiobiose + H2O 2.5% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. 2 beta-D-glucose
-
 ?
3.2.1.21 ginsenoside Rb1 + H2O 63% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. ? + beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Fusobacterium sp. p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 sophorose + H2O 144% of activity with p-nitrophenyl-beta-D-glucopyranoside Fusobacterium sp. 2 beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 tetramer 4 * 80000, SDS-PAGE Fusobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6
-
 at 37°C Fusobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 4 8.5 approx. 5% of maximal activity at pH 4.0 and pH 8.5, respectively Fusobacterium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Fusobacterium sp. chromatofocusing
-
 5.25