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Literature summary extracted from

  • Ohara, H.; Noguchi, J.; Karita, S.; Kimura, T.; Sakka, K.; Ohmiya, K.
    Sequence of egV and properties of EgV, a Ruminococcus albus endoglucanase containing a dockerin domain (2000), Biosci. Biotechnol. Biochem., 64, 80-88.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 truncated enzyme form purified from recombinant Escherichia coli Ruminococcus albus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 cell surface
-
 Ruminococcus albus 9986
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 42000
-
 x * 42000, truncated enzyme form expressed in Escherichia coli Ruminococcus albus
3.2.1.4 67103
-
 x * 67103, calculation from nucleotide sequence Ruminococcus albus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Ruminococcus albus Q9LCC5 F-40
-
3.2.1.4 Ruminococcus albus F-40 Q9LCC5 F-40
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Ruminococcus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 acid-swollen cellulose + H2O activity of truncated enzyme form Ruminococcus albus ?
-
 ?
3.2.1.4 acid-swollen cellulose + H2O activity of truncated enzyme form Ruminococcus albus F-40 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O activity of truncated enzyme form Ruminococcus albus ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O activity of truncated enzyme form Ruminococcus albus F-40 ?
-
 ?
3.2.1.4 lichenan + H2O activity of truncated enzyme form Ruminococcus albus ?
-
 ?
3.2.1.4 lichenan + H2O activity of truncated enzyme form Ruminococcus albus F-40 ?
-
 ?
3.2.1.4 xylan + H2O activity of truncated enzyme form Ruminococcus albus ?
-
 ?
3.2.1.4 xylan + H2O activity of truncated enzyme form Ruminococcus albus F-40 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 42000, truncated enzyme form expressed in Escherichia coli Ruminococcus albus
3.2.1.4 ? x * 67103, calculation from nucleotide sequence Ruminococcus albus
3.2.1.4 More modular enzyme composed of a catalytic domain of family 5 of glycosyl hydrolases, a domain of unknown function, and a dockerin domain responsible for cellulosome assembly. The enzyme is a component of the cellulosome Ruminococcus albus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 EgV
-
 Ruminococcus albus
3.2.1.4 endoglucanase V
-
 Ruminococcus albus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 40
-
 truncated enzyme form Ruminococcus albus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 40
-
 1 0 min, stable Ruminococcus albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 7
-
 truncated enzyme form Ruminococcus albus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 5 9 37°C, 30 min, stable Ruminococcus albus