EC Number | Cloned (Comment) | Organism |
---|---|---|
4.6.1.19 | overexpression of wild-type and mutant enzymes in Escherichia coli BL21(DE3) | Momordica charantia |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.6.1.19 | E84A | site-directed mutagenesis, decreased kcat, 90% reduced activity compared to the wild-type enzyme | Momordica charantia |
4.6.1.19 | H34A | site-directed mutagenesis, active site residue, inactive mutant | Momordica charantia |
4.6.1.19 | H83A | site-directed mutagenesis, decreased kcat, 80% reduced activity compared to the wild-type enzyme | Momordica charantia |
4.6.1.19 | H88A | site-directed mutagenesis, active site residue, inactive mutant | Momordica charantia |
4.6.1.19 | K87A | site-directed mutagenesis, decreased kcat, 45% reduced activity compared to the wild-type enzyme | Momordica charantia |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.6.1.19 | 2.47 | - |
CpU | wild-type enzyme | Momordica charantia | |
4.6.1.19 | 2.58 | - |
CpU | mutant K87A | Momordica charantia | |
4.6.1.19 | 8.31 | - |
CpU | mutant H83A | Momordica charantia | |
4.6.1.19 | 11.2 | - |
CpU | mutant E84A | Momordica charantia |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.6.1.19 | Momordica charantia | - |
bitter gourd | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.6.1.19 | RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] | residues His34, His 83, Glu84, Lys87, and His88 may be involved in catalysis | Momordica charantia |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.6.1.19 | seed | - |
Momordica charantia | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.6.1.19 | CpU + H2O | - |
Momordica charantia | ? | - |
? | |
4.6.1.19 | additional information | transphosphorylation | Momordica charantia | ? | - |
? | |
4.6.1.19 | RNA + H2O | from yeast | Momordica charantia | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.6.1.19 | More | enzyme belongs to the ribonuclease T2 family | Momordica charantia |
4.6.1.19 | ribonuclease MC1 | - |
Momordica charantia |
4.6.1.19 | RNase MC1 | - |
Momordica charantia |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.6.1.19 | additional information | - |
additional information | wild-type and mutant enzymes | Momordica charantia |