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Literature summary extracted from

  • Chatani, E.; Tanimizu, N.; Ueno, H.; Hayashi, R.
    Expression of soluble bovine pancreatic ribonuclease A in Pichia pastoris and its purification and characterization (2000), Biosci. Biotechnol. Biochem., 64, 2437-2444.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.18 expression in Pichia pastoris Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.18 N34A unglycosylated mutant Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.18 additional information
-
 cytidine-2',3'-cyclic monophosphate type I and II isoenzymes, almost identical values to those of commercial enzyme, pH 5.5, 25°C Bos taurus
4.6.1.18 additional information
-
 CpA type I and II isoenzymes, almost identical values to those of commercial enzyme, pH 5.5, 25°C Bos taurus
4.6.1.18 0.45
-
 cytidine-2',3'-cyclic monophosphate pH 5.5, 25ºC, comercial enzyme Bos taurus
4.6.1.18 0.46
-
 cytidine-2',3'-cyclic monophosphate pH 5.5, 25ºC, type I enzyme Bos taurus
4.6.1.18 0.5
-
 cytidinyl-3',5'-adenosine pH 5.5, 25ºC, comercial enzyme Bos taurus
4.6.1.18 0.52
-
 cytidinyl-3',5'-adenosine pH 5.5, 25ºC, type I enzyme Bos taurus
4.6.1.18 0.58
-
 cytidine-2',3'-cyclic monophosphate pH 5.5, 25ºC, N34A mutant Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.18 13640
-
 N34A mutant, mass spectrometry Bos taurus
4.6.1.18 13680
-
 type I isoenzyme, mass spectrometry Bos taurus
4.6.1.18 13850 15310 type II isoenzyme, multiple mass peaks, mass spectrometry Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus
-
 bovine
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.6.1.18 glycoprotein type II isoenzyme, O-linked glycoform Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.18 cation exchange chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.18 additional information
-
 type I isoform, 100% specific activity relative to commercial RNase A. Type II isoform, 53% specific activity relative to commercial enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 CpA + H2O
-
 Bos taurus adenosine + 3'-CMP
-
 ?
4.6.1.18 cytidine-2',3'-cyclic monophosphate + H2O hydrolysis reaction Bos taurus 3'-CMP
-
 ?
4.6.1.18 cytidinyl-3',5'-adenosine + H2O CpA, transphosphorylation reaction Bos taurus adenosine + 3'-CMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.18 type I RNase A isoform with the same molecular mass as that of a commercial RNase A Bos taurus
4.6.1.18 type II RNase A isoform with higher molecular mass than commercial and type I RNase A Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.18 additional information
-
 type I isoenzyme, Tm of 59°C. Type II isoenzyme, Tm of 53°C Bos taurus