Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Saraswat, V.; Bisaria, V.S.
    Purification, characterization and substrate specificities of xylanase isoenzymes from Melanocarpus albomyces IIS 68 (2000), Biosci. Biotechnol. Biochem., 64, 1173-1180.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 iodoacetic acid activation of isozymes Ic and IIB Melanocarpus albomyces

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ba2+ inhibition of isozyme Ic Melanocarpus albomyces
3.2.1.8 Co2+ inhibition of isozyme Ic Melanocarpus albomyces
3.2.1.8 Fe3+ inhibition of isozyme Ic Melanocarpus albomyces
3.2.1.8 Hg2+ inhibition of isozymes Ic and IIb Melanocarpus albomyces
3.2.1.8 Mn2+ inhibition of isozyme Ic Melanocarpus albomyces
3.2.1.8 N-bromosuccinimide inhibition of isozymes Ic and IIb Melanocarpus albomyces
3.2.1.8 SDS inhibition of isozyme IIb Melanocarpus albomyces

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information Km in mg/ml Melanocarpus albomyces

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Ba2+ activation of isozyme IIb Melanocarpus albomyces
3.2.1.8 Ca2+ activation of isozyme IIb Melanocarpus albomyces
3.2.1.8 Co2+ activation of isozyme IIb Melanocarpus albomyces
3.2.1.8 Mg2+ activation of isozymes Ic and IIb Melanocarpus albomyces
3.2.1.8 Mn2+ activation of isozyme IIb Melanocarpus albomyces
3.2.1.8 Ni2+ activation of isozymes Ic and IIb Melanocarpus albomyces

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 17100
-
 isozyme Ic, gel filtration Melanocarpus albomyces
3.2.1.8 18600
-
 1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE Melanocarpus albomyces
3.2.1.8 20500
-
 isozyme Ib, gel filtration Melanocarpus albomyces
3.2.1.8 20700
-
 1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE Melanocarpus albomyces
3.2.1.8 22900
-
 1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE Melanocarpus albomyces
3.2.1.8 23700
-
 isozyme Ia, gel filtration Melanocarpus albomyces
3.2.1.8 25100
-
 isozyme IIb, gel filtration Melanocarpus albomyces
3.2.1.8 25400
-
 1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE Melanocarpus albomyces
3.2.1.8 31300
-
 1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE Melanocarpus albomyces
3.2.1.8 31700
-
 isozyme IIa, gel filtration Melanocarpus albomyces
3.2.1.8 32200
-
 isozyme IId, gel filtration Melanocarpus albomyces
3.2.1.8 34300
-
 1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE Melanocarpus albomyces
3.2.1.8 38500
-
 1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE Melanocarpus albomyces
3.2.1.8 39800
-
 isozyme IIc, gel filtration Melanocarpus albomyces

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Melanocarpus albomyces
-
 isozymes: Ia, Ib, Ic, IIa, IIb, IIc, IId, only isozyme Ic is constitutive
-
3.2.1.8 Melanocarpus albomyces IIS 68
-
 isozymes: Ia, Ib, Ic, IIa, IIb, IIc, IId, only isozyme Ic is constitutive
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 7 isozymes to homogeneity by gel filtration and ion-exchange chromatography, overview Melanocarpus albomyces

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 additional information
-
 activity with different xylans, overview Melanocarpus albomyces
3.2.1.8 3.33
-
 isozyme IIa, substrate oat spelt xylan Melanocarpus albomyces
3.2.1.8 3.91
-
 isozyme IId, substrate oat spelt xylan Melanocarpus albomyces
3.2.1.8 6.31
-
 isozyme IIc, substrate oat spelt xylan Melanocarpus albomyces
3.2.1.8 11.68
-
 isozyme IIb, substrate oat spelt xylan Melanocarpus albomyces
3.2.1.8 36.58
-
 isozyme Ib, substrate oat spelt xylan Melanocarpus albomyces
3.2.1.8 48.74
-
 isozyme Ia, substrate oat spelt xylan Melanocarpus albomyces
3.2.1.8 142.7
-
 isozyme Ic, substrate oat spelt xylan Melanocarpus albomyces

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 4-nitrophenyl beta-D-xylopyranoside + H2O only isozyme IIa shows beta-xylosidase activity Melanocarpus albomyces 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.8 4-nitrophenyl beta-D-xylopyranoside + H2O only isozyme IIa shows beta-xylosidase activity Melanocarpus albomyces IIS 68 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.8 beechwood xylan + H2O
-
 Melanocarpus albomyces ?
-
 ?
3.2.1.8 beechwood xylan + H2O
-
 Melanocarpus albomyces IIS 68 ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 Melanocarpus albomyces ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 Melanocarpus albomyces IIS 68 ?
-
 ?
3.2.1.8 additional information substrate specificity for different xylans, overview, isozyme Ia, Ib, Ic, and IIa show also arabinose-releasing property on larchwood xylan Melanocarpus albomyces ?
-
 ?
3.2.1.8 additional information substrate specificity for different xylans, overview, isozyme Ia, Ib, Ic, and IIa show also arabinose-releasing property on larchwood xylan Melanocarpus albomyces IIS 68 ?
-
 ?
3.2.1.8 xylan + H2O substrate specificity of isozymes with different xylans: isozymes Ia, Ib, and Ic prefer larchwood arabinoglucurono xylan, isozymes IIa and IIc prefer birchwood glucurono xylan, isozyme IIb and IId prefer beechwood glucuronoxylan, another substrate is xylan from oat spelt, solka floc xylan is utilized only by isozyme Ic Melanocarpus albomyces xylooligosaccharides + D-xylose + xylobiose isozyme Ia produces only xylooligosaccharides ?
3.2.1.8 xylan + H2O substrate specificity of isozymes with different xylans: isozymes Ia, Ib, and Ic prefer larchwood arabinoglucurono xylan, isozymes IIa and IIc prefer birchwood glucurono xylan, isozyme IIb and IId prefer beechwood glucuronoxylan, another substrate is xylan from oat spelt, solka floc xylan is utilized only by isozyme Ic Melanocarpus albomyces IIS 68 xylooligosaccharides + D-xylose + xylobiose isozyme Ia produces only xylooligosaccharides ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 22900, isozyme Ia, SDS-PAGE, 1 * 20700, isozyme Ib, SDS-PAGE, 1 * 18600, isozyme Ic, SDS-PAGE, 1 * 31300, isozyme IIa, SDS-PAGE, 1 * 25400, isozyme IIb, SDS-PAGE, 1 * 38500, isozyme IIc, SDS-PAGE, 1 * 34300, isozyme IId, SDS-PAGE Melanocarpus albomyces

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Melanocarpus albomyces

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 55
-
 isozyme IIc Melanocarpus albomyces
3.2.1.8 60
-
 isozymes IIa and IIb Melanocarpus albomyces
3.2.1.8 65
-
 isozyme IId Melanocarpus albomyces
3.2.1.8 70
-
 isozymes Ia, Ib, and Ic Melanocarpus albomyces

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 additional information
-
 half-lives of the 7 isozymes at different temperatures, isozyme Ia is the most stable, overview Melanocarpus albomyces
3.2.1.8 60
-
 isozyme IIb shows a half-life of 94.5 min, isozyme IId of 85.6 min, isozyme Ia of 164.3 min Melanocarpus albomyces
3.2.1.8 70
-
 isozyme Ia shows a half-life of 16.7 min, isozyme IId of 15.6 min Melanocarpus albomyces
3.2.1.8 80
-
 all isozymes are inactivated within 10 min Melanocarpus albomyces

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 10.5
-
 birchwood xylan isozyme IIc, pH 6.0, 70°C Melanocarpus albomyces
3.2.1.8 10.7
-
 beechwood xylan isozyme IId, pH 6.0, 70°C Melanocarpus albomyces
3.2.1.8 13.9
-
 larchwood xylan isozyme Ia, pH 6.0, 70°C Melanocarpus albomyces
3.2.1.8 41.2
-
 larchwood xylan isozyme Ib, pH 6.0, 70°C Melanocarpus albomyces
3.2.1.8 53.4
-
 larchwood xylan isozyme Ic, pH 6.0, 70°C Melanocarpus albomyces
3.2.1.8 73
-
 birchwood xylan isozyme IIa, pH 6.0, 70°C Melanocarpus albomyces
3.2.1.8 83.8
-
 beechwood xylan isozyme IIb, pH 6.0, 70°C Melanocarpus albomyces

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5.5
-
 isozyme Ib Melanocarpus albomyces
3.2.1.8 6
-
 isozymes Ia and Ic Melanocarpus albomyces
3.2.1.8 7
-
 isozymes IIb and IId Melanocarpus albomyces
3.2.1.8 8
-
 isozyme IIc Melanocarpus albomyces
3.2.1.8 8.5
-
 isozyme IIa Melanocarpus albomyces

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Melanocarpus albomyces isozyme IIa, isoelectric focusing
-
 4.2
3.2.1.8 Melanocarpus albomyces isozyme IIb, isoelectric focusing
-
 4.2
3.2.1.8 Melanocarpus albomyces isozyme Ia, isoelectric focusing
-
 6
3.2.1.8 Melanocarpus albomyces isozyme Ib, isoelectric focusing
-
 6
3.2.1.8 Melanocarpus albomyces isozyme Ic, isoelectric focusing
-
 6