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Literature summary extracted from

  • Wakayama, M.; Yada, H.; Kanda, S.; Hayashi, S.; Yatsuda, Y.; Sakai, K.; Moriguchi, M.
    Role of conserved histidine residues in D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 (2000), Biosci. Biotechnol. Biochem., 64, 1-8.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.81 H250N KM-value for N-acetyl-D-leucine is 6fold higher than the wild-type value, turnover number is 0.0246% of the wild-type value. Below 1% of the relative activity compared with wild-type enzyme.The zinc content of the mutant enzyme is 0.7 gatom per mol, compared to 2.3 gatom per mol for the wild-type enzyme Achromobacter xylosoxidans
3.5.1.81 H251N approximately 30% of the activity of wild-type enzyme. KM-value for N-acetyl-D-leucine is 32% of the wild-type value, turnover number is 48% of the wild-type value. Achromobacter xylosoxidans
3.5.1.81 H67I no detectable activity Achromobacter xylosoxidans
3.5.1.81 H67N KM-value for N-acetyl-D-leucine is 75% of the wild-type value, turnover number is 0.0012% of the wild-type value. The zinc content of the mutant enzyme is 2.2 gatom per mol, compared to 2.3 gatom per mol for the wild-type enzyme Achromobacter xylosoxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.81 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide 16% inhibition at 1 mM, 45% inhibition at 10 mM Achromobacter xylosoxidans
3.5.1.81 2,4,6-Trinitrobenzene-1-sulfonate 40% inhihition at 1 mM, 86% inhibition at 10 mM Achromobacter xylosoxidans
3.5.1.81 3-Bromopyruvate 10 mM, 28% inhibition Achromobacter xylosoxidans
3.5.1.81 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride 17% inhibition at 1 mM, 42% inhibition at 10 mM Achromobacter xylosoxidans
3.5.1.81 5,5'-dithiobis(2-nitrobenzoic acid) 10 mM, 41% inhibition Achromobacter xylosoxidans
3.5.1.81 diethyl dicarbonate 93% inhibition by 93 mM, complete inhibition by 10 mM Achromobacter xylosoxidans
3.5.1.81 iodoacetate 10 mM, 37% inhibition Achromobacter xylosoxidans
3.5.1.81 N-Acetylimidazole 88% inhibition at 1 mM, 96% inhibition at 10 mM Achromobacter xylosoxidans
3.5.1.81 NEM 10 mM, 27% inhibition Achromobacter xylosoxidans
3.5.1.81 Phenylglyoxal 55% inhibition at 1 mM, 98% inhibition at 10 mM Achromobacter xylosoxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.81 0.61
-
 N-acetyl-D-leucine mutant enzyme H251N Achromobacter xylosoxidans
3.5.1.81 1.44
-
 N-acetyl-D-leucine mutant enzyme H67N Achromobacter xylosoxidans
3.5.1.81 1.93
-
 N-acetyl-D-leucine wild-type enzyme Achromobacter xylosoxidans
3.5.1.81 11.6
-
 N-acetyl-D-leucine mutant enzyme H250N Achromobacter xylosoxidans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.81 Zinc wild-type enzyme contains 2.3 gatom of zinc per mol of enzyme, mutant enzyme H67N contains 2.2 gatom of zinc per mol of enzyme, mutant enzyme H250N contains 0.7 gatom of zinc per mol of enzyme Achromobacter xylosoxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.81 52000
-
 x * 52000, wild-type enzyme and mutant enzymes H167N, H67I, H250N and H251N, SDS-PAGE Achromobacter xylosoxidans

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.81 Achromobacter xylosoxidans
-
 subsp. xylosoxydans A-6
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.81 N-acetyl-D-leucine + H2O
-
 Achromobacter xylosoxidans acetate + D-leucine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.81 ? x * 52000, wild-type enzyme and mutant enzymes H167N, H67I, H250N and H251N, SDS-PAGE Achromobacter xylosoxidans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.81 0.0147
-
 N-acetyl-D-leucine mutant enzyme H67N Achromobacter xylosoxidans
3.5.1.81 0.297
-
 N-acetyl-D-leucine mutant enzyme H250N Achromobacter xylosoxidans
3.5.1.81 593.3
-
 N-acetyl-D-leucine mutant enzyme H251N Achromobacter xylosoxidans
3.5.1.81 1225
-
 N-acetyl-D-leucine wild-type enzyme Achromobacter xylosoxidans