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Literature summary extracted from

  • Amitani, I.; Sakamoto, T.; Ando, T.
    Link between the enzymatic kinetics and mechanical behavior in an actomyosin motor (2001), Biophys. J., 80, 379-397.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.1.8 actin
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.1.8 0.00000027
-
 actin pH 7.6, 25°C, cosubstrate GTP Oryctolagus cuniculus
5.6.1.8 0.000008
-
 actin pH 7.6, 25°C, cosubstrate ITP Oryctolagus cuniculus
5.6.1.8 0.0000096
-
 actin pH 7.6, 25°C, presence of Sr2+ at 2 mM Oryctolagus cuniculus
5.6.1.8 0.000012
-
 actin pH 7.6, 25°C, cosubstrate TTP Oryctolagus cuniculus
5.6.1.8 0.000017
-
 actin pH 7.6, 25°C, presence of Ni2+ at 2 mM Oryctolagus cuniculus
5.6.1.8 0.000022
-
 actin pH 7.6, 25°C, presence of Mn2+ at 2 mM Oryctolagus cuniculus
5.6.1.8 0.000028
-
 actin pH 7.6, 25°C, cosubstrate CTP Oryctolagus cuniculus
5.6.1.8 0.000029
-
 actin pH 7.6, 25°C, cosubstrate ATP Oryctolagus cuniculus
5.6.1.8 0.000029
-
 actin pH 7.6, 25°C, presence of Mg2+ at 2 mM Oryctolagus cuniculus
5.6.1.8 0.00007
-
 actin pH 7.6, 25°C, cosubstrate UTP Oryctolagus cuniculus
5.6.1.8 0.0009
-
 ATP pH 7.6, 25°C Oryctolagus cuniculus
5.6.1.8 0.11
-
 CTP pH 7.6, 25°C Oryctolagus cuniculus
5.6.1.8 0.2
-
 UTP pH 7.6, 25°C Oryctolagus cuniculus
5.6.1.8 0.25
-
 TTP pH 7.6, 25°C Oryctolagus cuniculus
5.6.1.8 0.5
-
 GTP pH 7.6, 25°C Oryctolagus cuniculus
5.6.1.8 1
-
 ITP pH 7.6, 25°C Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.8 Mg2+ activates Oryctolagus cuniculus
5.6.1.8 Mn2+ activates Oryctolagus cuniculus
5.6.1.8 Ni2+ activates Oryctolagus cuniculus
5.6.1.8 Sr2+ activates Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.8 Oryctolagus cuniculus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.6.1.8 ATP + H2O + myosin bound to actin filament at position n = ADP + phosphate + myosin bound to actin filament at position n+1 kinetics and mechanical behaviour Oryctolagus cuniculus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.8 ATP + H2O + actin
-
 Oryctolagus cuniculus ADP + phosphate + actin
-
 ?
5.6.1.8 CTP + H2O + actin
-
 Oryctolagus cuniculus CDP + phosphate + actin
-
 ?
5.6.1.8 gTP + H2O + actin
-
 Oryctolagus cuniculus GDP + phosphate + actin
-
 ?
5.6.1.8 ITP + H2O + actin
-
 Oryctolagus cuniculus IDP + phosphate + actin
-
 ?
5.6.1.8 TTP + H2O + actin
-
 Oryctolagus cuniculus TDP + phosphate + actin
-
 ?
5.6.1.8 UTP + H2O + actin
-
 Oryctolagus cuniculus UDP + phosphate + actin
-
 ?