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Literature summary extracted from

  • Khunsook, S.; Bean, B.S.; McGowan, S.R.; Alhadeff, J.A.
    Purification and characterization of plasma membrane-associated human sperm alpha-L-fucosidase (2003), Biol. Reprod., 68, 709-716.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.51 In the presence of 0.015% Triton X-100 the frozen enzyme retains 100% activity after 3 months, in absence of 0.015% Triton X-100 the enzyme loses all activity after 2 weeks. In the presence or absence of 0.015% Triton X-100 and 2-4°C retains 100% activity after 2 weeks. Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.08
-
4-methylumbelliferyl alpha-L-fucopyranoside pH 7.0, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 51000
-
x * 51000, SDS-PAGE Homo sapiens
3.2.1.51 86000
-
major form Homo sapiens
3.2.1.51 230000
-
minor form Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.51 additional information treatment of enzyme with neuraminidase does not change its isoform profile Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 sperm plasma membrane associated Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 35
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.2.1.51 -20°C, in the presence of 1% fucose and presence or absence of 0.015% Triton X-100, 100% activity after 2 weeks Homo sapiens
3.2.1.51 2-4°C, in the presence of 1% fucose and 0.015% Triton X-100, 100% activity after 3 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-methylumbelliferyl alpha-L-fucopyranoside + H2O
-
Homo sapiens 4-methylumbelliferone + alpha-L-fucose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 ? x * 51000, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 6.8 7.3
-
Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.51 Homo sapiens isoelectric focusing, possible minor isoform
-
6
3.2.1.51 Homo sapiens isoelectric focusing, isoform I
-
6.5
3.2.1.51 Homo sapiens isoelectric focusing, isoform II
-
7.1