Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wild, C.; Golderer, G.; Grobner, P.; Werner-Felmayer, G.; Werner, E.R.
    Physarum polycephalum expresses a dihydropteridine reductase with selectivity for pterin substrates with a 6-(1', 2'-dihydroxypropyl) substitution (2003), Biol. Chem., 384, 1057-1062.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.34 recombinant expression in Escherichia coli Physarum polycephalum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.34 cytosol
-
 Physarum polycephalum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.34 23000
-
 2 * 23000, about, recombinant enzyme, SDS-PAGE Physarum polycephalum
1.5.1.34 46000
-
 about, recombinant enzyme, gel filtration Physarum polycephalum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.34 5,6,7,8-tetrahydropteridine + NAD(P)+ Physarum polycephalum enzyme is required for recycling of 6,7-dihydropteridine, in vivo regulation is related to growth phase 6,7-dihydropteridine + NAD(P)H
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Physarum polycephalum Q8WTJ2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.34 recombinant enzyme from Escherichia coli by ion exchange chromatography and gel filtration to homogeneity Physarum polycephalum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.34 microplasmodium
-
 Physarum polycephalum
-
1.5.1.34 additional information constant enzyme expression during cell cycle of multinuclear microplasmodia, but down-regulation during development of sporangia, inhibited or reversed by glucose feeding, expression profile Physarum polycephalum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.34 additional information
-
-
 Physarum polycephalum
1.5.1.34 0.069
-
 enzyme extract of microplasmodia Physarum polycephalum
1.5.1.34 38.3
-
 purified recombinant enzyme Physarum polycephalum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 (6R)-tetrahydrobiopterin + NAD(P)+
-
 Physarum polycephalum ?
-
 r
1.5.1.34 (6R,S)-6-hydroxy-methyltetrahydropterin + NAD(P)+ low activity Physarum polycephalum ?
-
 r
1.5.1.34 (6R,S)-6-methyl-tetrahydropterin + NAD(P)+ low activity Physarum polycephalum ?
-
 r
1.5.1.34 (6R,S)-tetrahydrodictyopterin + NAD(P)+
-
 Physarum polycephalum ?
-
 r
1.5.1.34 (6R,S)-tetrahydrofolic acid + NAD(P)+ low activity Physarum polycephalum ?
-
 r
1.5.1.34 (6R,S)-tetrahydroneopterin + NAD(P)+ low activity Physarum polycephalum ?
-
 r
1.5.1.34 (6S)-tetrahydrobiopterin + NAD(P)+ low activity Physarum polycephalum ?
-
 r
1.5.1.34 5,6,7,8-tetrahydropteridine + NAD(P)+ enzyme is required for recycling of 6,7-dihydropteridine, in vivo regulation is related to growth phase Physarum polycephalum 6,7-dihydropteridine + NAD(P)H
-
 r
1.5.1.34 6,7-dimethyl-tetrahydropterin + NAD(P)+ low activity Physarum polycephalum ?
-
 r
1.5.1.34 additional information substrate specificity, enzyme shows selectivity for pterin substrates with a 6-(1', 2'-dihydroxypropyl) substitution Physarum polycephalum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.34 dimer 2 * 23000, about, recombinant enzyme, SDS-PAGE Physarum polycephalum

Synonyms

EC Number Synonyms Comment Organism
1.5.1.34 DHPR
-
 Physarum polycephalum
1.5.1.34 dihydropteridine reductase
-
 Physarum polycephalum
1.5.1.34 quinoid dihydropteridine reductase
-
 Physarum polycephalum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 NAD(P)H NADH is the preferred cofactor Physarum polycephalum