BRENDA - Enzyme Database

Effect of metabolites of gamma-aminobutyric shunt on activities of NAD- and NADP-isocitrate dehydrogenases and aconitate hydratase from higher plants

Pinheiro de Carvalho, M.A.A.; Popova, T.N.; Dos Santos, T.M.M.; Zherebtsov, N.A.; Biol. Bull. 30, 236-242 (2003)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.41
additional information
enzyme is not affected by gamma-aminobutyric acid
Zea mays
1.1.1.42
glutamate
-
Pisum sativum
1.1.1.42
glutamate
-
Zea mays
1.1.1.42
additional information
enzyme is not affected by gamma-aminobutyric acid and succinate
Pisum sativum
1.1.1.42
additional information
enzyme is not affected by gamma-aminobutyric acid and succinate
Zea mays
1.1.1.42
succinic acid
-
Pisum sativum
1.1.1.42
succinic acid
-
Zea mays
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
gamma-aminobutyric acid
slight inhibition
Pisum sativum
1.1.1.41
glutamate
slight inhibition
Pisum sativum
1.1.1.41
additional information
enzyme is not affected by gamma-aminobutyric acid and glutamate
Zea mays
1.1.1.41
succinate
slight inhibition
Pisum sativum
1.1.1.41
succinate
slight inhibition
Zea mays
1.1.1.41
Succinic semialdehyde
slight inhibition
Pisum sativum
1.1.1.41
Succinic semialdehyde
slight inhibition
Zea mays
1.1.1.42
additional information
enzyme is not affected by gamma-aminobutyric acid and succinate
Pisum sativum
1.1.1.42
additional information
enzyme is not affected by gamma-aminobutyric acid and succinate
Zea mays
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
exclusively
Pisum sativum
5739
-
1.1.1.42
cytosol
-
Pisum sativum
5829
-
1.1.1.42
cytosol
-
Zea mays
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mn2+
-
Pisum sativum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Pisum sativum
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Zea mays
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.42
isocitrate + NADP+
Pisum sativum
-
2-oxoglutarate + CO2 + NADPH
-
-
?
1.1.1.42
isocitrate + NADP+
Zea mays
-
2-oxoglutarate + CO2 + NADPH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Pisum sativum
-
-
-
1.1.1.41
Zea mays
-
-
-
1.1.1.42
Pisum sativum
-
-
-
1.1.1.42
Zea mays
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
leaf
-
Pisum sativum
-
1.1.1.41
scutellum
-
Zea mays
-
1.1.1.42
leaf
-
Pisum sativum
-
1.1.1.42
scutellum
-
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.41
1.7
-
-
Pisum sativum
1.1.1.41
2.9
-
-
Zea mays
1.1.1.42
27.2
-
purified enzyme
Pisum sativum
1.1.1.42
34.6
-
purified enzyme
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
654914
Pisum sativum
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
654914
Zea mays
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
654914
Pisum sativum
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
654914
Zea mays
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.41
25
-
assay at
Pisum sativum
1.1.1.41
25
-
assay at
Zea mays
1.1.1.42
25
-
assay at
Pisum sativum
1.1.1.42
25
-
assay at
Zea mays
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
specific for
Pisum sativum
1.1.1.41
NAD+
specific for
Zea mays
1.1.1.42
NADP+
specific for
Pisum sativum
1.1.1.42
NADP+
specific for
Zea mays
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.41
additional information
enzyme is not affected by gamma-aminobutyric acid
Zea mays
1.1.1.42
glutamate
-
Pisum sativum
1.1.1.42
glutamate
-
Zea mays
1.1.1.42
additional information
enzyme is not affected by gamma-aminobutyric acid and succinate
Pisum sativum
1.1.1.42
additional information
enzyme is not affected by gamma-aminobutyric acid and succinate
Zea mays
1.1.1.42
succinic acid
-
Pisum sativum
1.1.1.42
succinic acid
-
Zea mays
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
specific for
Pisum sativum
1.1.1.41
NAD+
specific for
Zea mays
1.1.1.42
NADP+
specific for
Pisum sativum
1.1.1.42
NADP+
specific for
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
gamma-aminobutyric acid
slight inhibition
Pisum sativum
1.1.1.41
glutamate
slight inhibition
Pisum sativum
1.1.1.41
additional information
enzyme is not affected by gamma-aminobutyric acid and glutamate
Zea mays
1.1.1.41
succinate
slight inhibition
Pisum sativum
1.1.1.41
succinate
slight inhibition
Zea mays
1.1.1.41
Succinic semialdehyde
slight inhibition
Pisum sativum
1.1.1.41
Succinic semialdehyde
slight inhibition
Zea mays
1.1.1.42
additional information
enzyme is not affected by gamma-aminobutyric acid and succinate
Pisum sativum
1.1.1.42
additional information
enzyme is not affected by gamma-aminobutyric acid and succinate
Zea mays
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
exclusively
Pisum sativum
5739
-
1.1.1.42
cytosol
-
Pisum sativum
5829
-
1.1.1.42
cytosol
-
Zea mays
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mn2+
-
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Pisum sativum
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Zea mays
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.42
isocitrate + NADP+
Pisum sativum
-
2-oxoglutarate + CO2 + NADPH
-
-
?
1.1.1.42
isocitrate + NADP+
Zea mays
-
2-oxoglutarate + CO2 + NADPH
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
leaf
-
Pisum sativum
-
1.1.1.41
scutellum
-
Zea mays
-
1.1.1.42
leaf
-
Pisum sativum
-
1.1.1.42
scutellum
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.41
1.7
-
-
Pisum sativum
1.1.1.41
2.9
-
-
Zea mays
1.1.1.42
27.2
-
purified enzyme
Pisum sativum
1.1.1.42
34.6
-
purified enzyme
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
654914
Pisum sativum
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
654914
Zea mays
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
654914
Pisum sativum
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
654914
Zea mays
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.41
25
-
assay at
Pisum sativum
1.1.1.41
25
-
assay at
Zea mays
1.1.1.42
25
-
assay at
Pisum sativum
1.1.1.42
25
-
assay at
Zea mays