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Literature summary extracted from

  • Schultz, A.; Baltscheffsky, M.
    Inhibition studies on Rhodospirillum rubrum H(+)-pyrophosphatase expressed in Escherichia coli (2004), Biochim. Biophys. Acta, 1656, 156-165.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.3.1 expressed in Escherichia coli C43(DE3) Rhodospirillum rubrum

Protein Variants

EC Number Protein Variants Comment Organism
7.1.3.1 D204H/R206L 90% loss in proton translocating but only 50% loss in hydrolytic activity Rhodospirillum rubrum
7.1.3.1 D217A almost complete loss of hydrolytic and proton translocating activity, less Mg2+ required for activity Rhodospirillum rubrum
7.1.3.1 D217H almost complete loss of hydrolytic and proton translocating activity, less Mg2+ required for activity Rhodospirillum rubrum
7.1.3.1 H372A slight loss of activity Rhodospirillum rubrum
7.1.3.1 H372A/H702V slight loss of activity Rhodospirillum rubrum
7.1.3.1 H469R almost complete loss of hydrolytic and proton translocating activity Rhodospirillum rubrum
7.1.3.1 H652A slight loss of activity Rhodospirillum rubrum
7.1.3.1 H661A about 50% loss of activity Rhodospirillum rubrum
7.1.3.1 H702V slight loss of activity Rhodospirillum rubrum

General Stability

EC Number General Stability Organism
7.1.3.1 Mg2+/diphosphate protects against inhibition by all inhibitors in the study Rhodospirillum rubrum

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.3.1 4-bromophenacyl bromide
-
Rhodospirillum rubrum
7.1.3.1 diethylpyrocarbonate
-
Rhodospirillum rubrum
7.1.3.1 Fluorescein 5'-isothiocyanate
-
Rhodospirillum rubrum
7.1.3.1 N,N'-dicyclohexyl-carbodiimide
-
Rhodospirillum rubrum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.3.1 membrane bound Rhodospirillum rubrum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.3.1 Mg2+ required Rhodospirillum rubrum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.3.1 60000
-
SDS-PAGE Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.3.1 diphosphate + H2O + H+[side 1] Rhodospirillum rubrum important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation 2 phosphate + H+[side 2]
-
?

Organism

EC Number Organism UniProt Comment Textmining
7.1.3.1 Rhodospirillum rubrum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.3.1 diphosphate + H2O + H+[side 1]
-
Rhodospirillum rubrum 2 phosphate + H+[side 2]
-
?
7.1.3.1 diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation Rhodospirillum rubrum 2 phosphate + H+[side 2]
-
?