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Literature summary extracted from

  • Yin, X.; Proteau, P.J.
    Characterization of native and histidine-tagged deoxyxylulose 5-phosphate reductoisomerase from the cyanobacterium Synechocystis sp. PCC6803 (2003), Biochim. Biophys. Acta, 1652, 75-81.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 NaCl 100 mM, 87% residual activity Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 0.001
-
NADPH pH 7.5, 37°C, presence of 1 mM Co2+ Synechocystis sp.
1.1.1.267 0.0033
-
NADPH pH 7.5, 37°C, presence of 1 mM Mn2+ Synechocystis sp.
1.1.1.267 0.005
-
NADPH pH 7.5, 37°C, presence of 1 mM Mg2+ Synechocystis sp.
1.1.1.267 0.063
-
1-deoxy-D-xylulose-5-phosphate pH 7.5, 37°C, presence of 1 mM Co2+ Synechocystis sp.
1.1.1.267 0.134
-
1-deoxy-D-xylulose-5-phosphate pH 7.5, 37°C, presence of 1 mM Mg2+ Synechocystis sp.
1.1.1.267 0.195
-
1-deoxy-D-xylulose-5-phosphate pH 7.5, 37°C, presence of 1 mM Mn2+ Synechocystis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Co2+ required, Km-value 0.001 mM Synechocystis sp.
1.1.1.267 Mg2+ Km-value 2.37 mM Synechocystis sp.
1.1.1.267 Mn2+ may substitute for Co2+, Km-value 0.0015 mM Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.267 42500
-
x * 42500, SDS-PAGE Synechocystis sp.
1.1.1.267 75000
-
PAGE Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Synechocystis sp.
-
strain PCC6803, native and recombinant enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose-5-phosphate + NADPH
-
Synechocystis sp. methyl-D-erythritol-4-phosphate + NADP+
-
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Subunits

EC Number Subunits Comment Organism
1.1.1.267 dimer x * 42500, SDS-PAGE Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 55
-
-
Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7.5 8
-
Synechocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADPH
-
Synechocystis sp.