BRENDA - Enzyme Database

Regulation of NAD- and NADP-dependent isocitrate dehydrogenases by reduction levels of pyridine nucleotides in mitochondria and cytosol of pea leaves

Igamberdiev, A.U.; Gardestrom, P.; Biochim. Biophys. Acta 1606, 117-125 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.41
additional information
the enzyme is not affected by NADP+, HCO3-, and 2-oxoglutarate
Pisum sativum
1.1.1.42
additional information
the mitochondrial isozyme is light-inducible, while the cytosolic one is not
Pisum sativum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
additional information
the enzyme is not affected by NADP+, HCO3-, and 2-oxoglutarate
Pisum sativum
1.1.1.41
NADH
competitive
Pisum sativum
1.1.1.41
NADPH
noncompetitive
Pisum sativum
1.1.1.42
2-oxoglutarate
-
Pisum sativum
1.1.1.42
isocitrate
reverse reaction
Pisum sativum
1.1.1.42
additional information
poor inhibition of the forward reaction by 2-oxoglutarate, no inhibition by CO2
Pisum sativum
1.1.1.42
NADH
noncompetitive
Pisum sativum
1.1.1.42
NADP+
reverse reaction
Pisum sativum
1.1.1.42
NADPH
forward reaction
Pisum sativum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.12
-
isocitrate
pH 7.5
Pisum sativum
1.1.1.41
0.2
-
NAD+
pH 7.5
Pisum sativum
1.1.1.42
0.003
-
NADP+
mitochondrial isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.004
-
isocitrate
mitochondrial isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.004
-
NADP+
cytosolic isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.006
-
isocitrate
cytosolic isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.009
-
NADPH
mitochondrial isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.017
-
2-oxoglutarate
mitochondrial isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.024
-
NADPH
cytosolic isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.026
-
2-oxoglutarate
cytosolic isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.2
-
CO2
mitochondrial isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
1.6
-
CO2
cytosolic isozyme, reverse reaction, pH 7.0
Pisum sativum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Pisum sativum
5739
-
1.1.1.42
cytosol
-
Pisum sativum
5829
-
1.1.1.42
mitochondrion
light-inducible isozyme
Pisum sativum
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mn2+
-
Pisum sativum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Pisum sativum
pyridine nucleotide contents in mitochondria and cytosol, regulation, overview
2-oxoglutarate + CO2 + NADH
-
-
ir
1.1.1.42
isocitrate + NADP+
Pisum sativum
the cytosolic isozyme supplies 2-oxoglutarate for the photorespiratory ammonia fixation, pyridine nucleotide contents in mitochondria and cytosol, regulation, overview
2-oxoglutarate + CO2 + NADPH
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Pisum sativum
-
cv. 294 040 Märgärt Kelvedon Wonder
-
1.1.1.42
Pisum sativum
-
cv. 294 040 Märgärt Kelvedon Wonder, one cytosolic and one mitochondrial isozyme
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.41
partially by mitochondrion isolation in a Percoll gradient, and ammonium sulfate precipitation in the 60-80% fraction, DEAE cellulose chromatography, and desalting
Pisum sativum
1.1.1.42
cytosolic and mitochondrial isozymes
Pisum sativum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
leaf
green and etiolated
Pisum sativum
-
1.1.1.42
leaf
green and etiolated
Pisum sativum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.41
0.084
-
mitochondria from green leaves, forward reaction
Pisum sativum
1.1.1.41
0.096
-
mitochondria from etiolated leaves, forward reaction
Pisum sativum
1.1.1.42
0.038
-
mitochondria from etiolated leaves, forward reaction at pH 7.5
Pisum sativum
1.1.1.42
0.072
-
mitochondria from green leaves, forward reaction at pH 7.5
Pisum sativum
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.41
-80°C, partially purified enzyme, several months, stable
Pisum sativum
1.1.1.42
-80°C, partially purified cytosolic and mitochondrial isozymes, several months, stable
Pisum sativum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
pyridine nucleotide contents in mitochondria and cytosol, regulation, overview
654843
Pisum sativum
2-oxoglutarate + CO2 + NADH
-
-
-
ir
1.1.1.41
threo-Ds-isocitrate + NAD+
-
654843
Pisum sativum
2-oxoglutarate + CO2 + NADH
-
-
-
ir
1.1.1.42
isocitrate + NADP+
-
654843
Pisum sativum
2-oxoglutarate + CO2 + NADPH
-
-
-
r
1.1.1.42
isocitrate + NADP+
the cytosolic isozyme supplies 2-oxoglutarate for the photorespiratory ammonia fixation, pyridine nucleotide contents in mitochondria and cytosol, regulation, overview
654843
Pisum sativum
2-oxoglutarate + CO2 + NADPH
-
-
-
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.5
-
-
Pisum sativum
1.1.1.42
6.3
6.5
reverse reaction, cytosolic isozyme
Pisum sativum
1.1.1.42
6.8
7
reverse reaction, mitochondrial isozyme
Pisum sativum
1.1.1.42
8
9
forward reaction, cytosolic and mitochondrial isozymes
Pisum sativum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
specific for
Pisum sativum
1.1.1.42
additional information
no activity with NAD+ and NADH
Pisum sativum
1.1.1.42
NADP+
absolutely specific for, forward reaction
Pisum sativum
1.1.1.42
NADPH
absolutely specific for, reverse reaction
Pisum sativum
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.41
0.18
-
NADH
pH 7.5
Pisum sativum
1.1.1.41
0.26
-
NADPH
pH 7.5
Pisum sativum
1.1.1.42
0.005
-
isocitrate
mitochondrial isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.005
-
NADPH
mitochondrial isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.006
-
NADP+
mitochondrial isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.007
-
NADPH
cytosolic isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.012
-
isocitrate
cytosolic isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.021
-
NADP+
cytosolic isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.06
-
NADH
mitochondrial isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.08
-
NADH
cytosolic isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
1
-
2-oxoglutarate
above, cytosolic isozyme, forward reaction, pH 8.0; above, mitochondrial isozyme, forward reaction, pH 8.0
Pisum sativum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.41
additional information
the enzyme is not affected by NADP+, HCO3-, and 2-oxoglutarate
Pisum sativum
1.1.1.42
additional information
the mitochondrial isozyme is light-inducible, while the cytosolic one is not
Pisum sativum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
specific for
Pisum sativum
1.1.1.42
additional information
no activity with NAD+ and NADH
Pisum sativum
1.1.1.42
NADP+
absolutely specific for, forward reaction
Pisum sativum
1.1.1.42
NADPH
absolutely specific for, reverse reaction
Pisum sativum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
additional information
the enzyme is not affected by NADP+, HCO3-, and 2-oxoglutarate
Pisum sativum
1.1.1.41
NADH
competitive
Pisum sativum
1.1.1.41
NADPH
noncompetitive
Pisum sativum
1.1.1.42
2-oxoglutarate
-
Pisum sativum
1.1.1.42
isocitrate
reverse reaction
Pisum sativum
1.1.1.42
additional information
poor inhibition of the forward reaction by 2-oxoglutarate, no inhibition by CO2
Pisum sativum
1.1.1.42
NADH
noncompetitive
Pisum sativum
1.1.1.42
NADP+
reverse reaction
Pisum sativum
1.1.1.42
NADPH
forward reaction
Pisum sativum
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.41
0.18
-
NADH
pH 7.5
Pisum sativum
1.1.1.41
0.26
-
NADPH
pH 7.5
Pisum sativum
1.1.1.42
0.005
-
isocitrate
mitochondrial isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.005
-
NADPH
mitochondrial isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.006
-
NADP+
mitochondrial isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.007
-
NADPH
cytosolic isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.012
-
isocitrate
cytosolic isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.021
-
NADP+
cytosolic isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.06
-
NADH
mitochondrial isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.08
-
NADH
cytosolic isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
1
-
2-oxoglutarate
above, cytosolic isozyme, forward reaction, pH 8.0; above, mitochondrial isozyme, forward reaction, pH 8.0
Pisum sativum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.12
-
isocitrate
pH 7.5
Pisum sativum
1.1.1.41
0.2
-
NAD+
pH 7.5
Pisum sativum
1.1.1.42
0.003
-
NADP+
mitochondrial isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.004
-
isocitrate
mitochondrial isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.004
-
NADP+
cytosolic isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.006
-
isocitrate
cytosolic isozyme, forward reaction, pH 8.0
Pisum sativum
1.1.1.42
0.009
-
NADPH
mitochondrial isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.017
-
2-oxoglutarate
mitochondrial isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.024
-
NADPH
cytosolic isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.026
-
2-oxoglutarate
cytosolic isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
0.2
-
CO2
mitochondrial isozyme, reverse reaction, pH 7.0
Pisum sativum
1.1.1.42
1.6
-
CO2
cytosolic isozyme, reverse reaction, pH 7.0
Pisum sativum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Pisum sativum
5739
-
1.1.1.42
cytosol
-
Pisum sativum
5829
-
1.1.1.42
mitochondrion
light-inducible isozyme
Pisum sativum
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mn2+
-
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Pisum sativum
pyridine nucleotide contents in mitochondria and cytosol, regulation, overview
2-oxoglutarate + CO2 + NADH
-
-
ir
1.1.1.42
isocitrate + NADP+
Pisum sativum
the cytosolic isozyme supplies 2-oxoglutarate for the photorespiratory ammonia fixation, pyridine nucleotide contents in mitochondria and cytosol, regulation, overview
2-oxoglutarate + CO2 + NADPH
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
partially by mitochondrion isolation in a Percoll gradient, and ammonium sulfate precipitation in the 60-80% fraction, DEAE cellulose chromatography, and desalting
Pisum sativum
1.1.1.42
cytosolic and mitochondrial isozymes
Pisum sativum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
leaf
green and etiolated
Pisum sativum
-
1.1.1.42
leaf
green and etiolated
Pisum sativum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.41
0.084
-
mitochondria from green leaves, forward reaction
Pisum sativum
1.1.1.41
0.096
-
mitochondria from etiolated leaves, forward reaction
Pisum sativum
1.1.1.42
0.038
-
mitochondria from etiolated leaves, forward reaction at pH 7.5
Pisum sativum
1.1.1.42
0.072
-
mitochondria from green leaves, forward reaction at pH 7.5
Pisum sativum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.41
-80°C, partially purified enzyme, several months, stable
Pisum sativum
1.1.1.42
-80°C, partially purified cytosolic and mitochondrial isozymes, several months, stable
Pisum sativum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
pyridine nucleotide contents in mitochondria and cytosol, regulation, overview
654843
Pisum sativum
2-oxoglutarate + CO2 + NADH
-
-
-
ir
1.1.1.41
threo-Ds-isocitrate + NAD+
-
654843
Pisum sativum
2-oxoglutarate + CO2 + NADH
-
-
-
ir
1.1.1.42
isocitrate + NADP+
-
654843
Pisum sativum
2-oxoglutarate + CO2 + NADPH
-
-
-
r
1.1.1.42
isocitrate + NADP+
the cytosolic isozyme supplies 2-oxoglutarate for the photorespiratory ammonia fixation, pyridine nucleotide contents in mitochondria and cytosol, regulation, overview
654843
Pisum sativum
2-oxoglutarate + CO2 + NADPH
-
-
-
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.5
-
-
Pisum sativum
1.1.1.42
6.3
6.5
reverse reaction, cytosolic isozyme
Pisum sativum
1.1.1.42
6.8
7
reverse reaction, mitochondrial isozyme
Pisum sativum
1.1.1.42
8
9
forward reaction, cytosolic and mitochondrial isozymes
Pisum sativum