BRENDA - Enzyme Database show

Substrate recognition by 2-oxoacid:ferredoxin oxidoreductase from Sulfolobus sp. strain 7

Fukuda, E.; Wakagi, T.; Biochim. Biophys. Acta 1597, 74-80 (2002)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.11
additional information
-
additional information
kinetic parameters of mutant enzymes
Sulfolobus sp.
1.2.7.11
0.28
-
pyruvate
pH 7.5, 80°C, natural and recombinant wild-type enzyme
Sulfolobus sp.
1.2.7.11
0.45
-
2-oxobutyrate
pH 7.5, 80°C, recombinant wild-type enzyme
Sulfolobus sp.
1.2.7.11
0.48
-
2-oxobutyrate
pH 7.5, 80°C, natural enzyme
Sulfolobus sp.
1.2.7.11
0.85
-
2-oxoglutarate
pH 7.5, 80°C, recombinant wild-type enzyme
Sulfolobus sp.
1.2.7.11
0.87
-
2-oxoglutarate
pH 7.5, 80°C, natural enzyme
Sulfolobus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.11
Sulfolobus sp.
-
-
-
1.2.7.11
Sulfolobus sp. 7
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.11
2-oxobutyrate + CoA + oxidized ferredoxin
kcat/Km for 2-oxobutyrate is 60% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme
654838
Sulfolobus sp.
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.11
2-oxobutyrate + CoA + oxidized ferredoxin
kcat/Km for 2-oxobutyrate is 60% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme
654838
Sulfolobus sp. 7
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.11
2-oxoglutarate + CoA + 2 oxidized ferredoxin
kcat/Km for 2-oxoglutarate is 12% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme
654838
Sulfolobus sp.
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
2-oxoglutarate + CoA + 2 oxidized ferredoxin
kcat/Km for 2-oxoglutarate is 12% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme
654838
Sulfolobus sp. 7
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
pyruvate + CoA + 2 oxidized ferredoxin
-
654838
Sulfolobus sp.
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
pyruvate + CoA + 2 oxidized ferredoxin
-
654838
Sulfolobus sp. 7
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.11
80
-
assay at
Sulfolobus sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.11
7.5
-
assay at
Sulfolobus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.11
additional information
-
additional information
kinetic parameters of mutant enzymes
Sulfolobus sp.
1.2.7.11
0.28
-
pyruvate
pH 7.5, 80°C, natural and recombinant wild-type enzyme
Sulfolobus sp.
1.2.7.11
0.45
-
2-oxobutyrate
pH 7.5, 80°C, recombinant wild-type enzyme
Sulfolobus sp.
1.2.7.11
0.48
-
2-oxobutyrate
pH 7.5, 80°C, natural enzyme
Sulfolobus sp.
1.2.7.11
0.85
-
2-oxoglutarate
pH 7.5, 80°C, recombinant wild-type enzyme
Sulfolobus sp.
1.2.7.11
0.87
-
2-oxoglutarate
pH 7.5, 80°C, natural enzyme
Sulfolobus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.11
2-oxobutyrate + CoA + oxidized ferredoxin
kcat/Km for 2-oxobutyrate is 60% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme
654838
Sulfolobus sp.
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.11
2-oxobutyrate + CoA + oxidized ferredoxin
kcat/Km for 2-oxobutyrate is 60% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme
654838
Sulfolobus sp. 7
propanoyl-CoA + CO2 + reduced ferredoxin
-
-
-
?
1.2.7.11
2-oxoglutarate + CoA + 2 oxidized ferredoxin
kcat/Km for 2-oxoglutarate is 12% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme
654838
Sulfolobus sp.
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
2-oxoglutarate + CoA + 2 oxidized ferredoxin
kcat/Km for 2-oxoglutarate is 12% compared to the kcat/Km-value for pyruvate, recombinant wild-type enzyme
654838
Sulfolobus sp. 7
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
pyruvate + CoA + 2 oxidized ferredoxin
-
654838
Sulfolobus sp.
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
pyruvate + CoA + 2 oxidized ferredoxin
-
654838
Sulfolobus sp. 7
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.11
80
-
assay at
Sulfolobus sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.11
7.5
-
assay at
Sulfolobus sp.