Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stubbs, M.D.; Tran, H.T.; Atwell, A.J.; Smith, C.S.; Olson, D.; Moorhead, G.B.
    Purification and properties of Arabidopsis thaliana type 1 protein phosphatase (PP1) (2001), Biochim. Biophys. Acta, 1550, 52-63.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 calyculin IC50: 0.09 nM Arabidopsis thaliana
3.1.3.16 cantharidin IC50: 60 nM Arabidopsis thaliana
3.1.3.16 diphosphate IC50: 0.07 mM Arabidopsis thaliana
3.1.3.16 fostriecin IC50: 0.022 mM Arabidopsis thaliana
3.1.3.16 microcystin-LR IC50: 0.01 nM Arabidopsis thaliana
3.1.3.16 NaF IC50: 2.1 mM Arabidopsis thaliana
3.1.3.16 nodularin IC50: 0.04 nM Arabidopsis thaliana
3.1.3.16 okadaic acid IC50: 20 nM Arabidopsis thaliana
3.1.3.16 phosphate IC50: 9.5 mM Arabidopsis thaliana
3.1.3.16 protein phosphatase inhibitor-2 IC50: 0.65 nM Arabidopsis thaliana
3.1.3.16 tautomycin IC50: 0.06 nM Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.16 35000
-
 1 * 35000 Da, SDS-PAGE Arabidopsis thaliana
3.1.3.16 39000
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16 purification of the catalytic and the regulatory subunit Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.16 8.95
-
-
 Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
3.1.3.16 -20°C, at least 2 months Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 phosphorylated glycogen phosphorylase alpha + H2O
-
 Arabidopsis thaliana glycogen phosphorylase alpha + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.16 monomer 1 * 35000 Da, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 PP1
-
 Arabidopsis thaliana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.16 0.00000001
-
 IC50: 0.01 nM Arabidopsis thaliana microcystin-LR
3.1.3.16 0.00000004
-
 IC50: 0.04 nM Arabidopsis thaliana nodularin
3.1.3.16 0.00000006
-
 IC50: 0.06 nM Arabidopsis thaliana tautomycin
3.1.3.16 0.00000009
-
 IC50: 0.09 nM Arabidopsis thaliana calyculin
3.1.3.16 0.00000065
-
 IC50: 0.65 nM Arabidopsis thaliana protein phosphatase inhibitor-2
3.1.3.16 0.00002
-
 IC50: 20 nM Arabidopsis thaliana okadaic acid
3.1.3.16 0.00006
-
 IC50: 60 nM Arabidopsis thaliana cantharidin
3.1.3.16 0.022
-
 IC50: 0.022 mM Arabidopsis thaliana fostriecin
3.1.3.16 0.07
-
 IC50: 0.07 mM Arabidopsis thaliana diphosphate
3.1.3.16 2.1
-
 IC50: 2.1 mM Arabidopsis thaliana NaF
3.1.3.16 9.5
-
 IC50: 9.5 mM Arabidopsis thaliana phosphate