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Literature summary extracted from

  • Bonnin, E.; Le Goff, A.; Korner, R.; Van Alebeek, G.W.; Christensen, T.M.; Voragen, A.G.; Roepstorff, P.; Caprari, C.; Thibault, J.
    Study of the mode of action of endopolygalacturonase from Fusarium moniliforme (2001), Biochim. Biophys. Acta, 1526, 301-309.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.15 additional information
-
 additional information the KM-value for homogalacturonan is 0.16 mg/ml Fusarium verticillioides
3.2.1.15 0.000016
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Fusarium verticillioides
3.2.1.15 0.000023
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Fusarium verticillioides
3.2.1.15 0.000052
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Fusarium verticillioides
3.2.1.15 0.000064
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Fusarium verticillioides
3.2.1.15 0.000071
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Fusarium verticillioides

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Fusarium verticillioides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15
-
 Fusarium verticillioides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.15 culture medium of transformed Saccharomyces cerevisae Fusarium verticillioides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 additional information
-
-
 Fusarium verticillioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Fusarium verticillioides alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + galacturonate
-
 ?
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Fusarium verticillioides alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + galacturonate
-
 ?
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Fusarium verticillioides alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + galacturonate no production of pentagalacturonate ?
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Fusarium verticillioides alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + galacturonate
-
 ?
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Fusarium verticillioides alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + galacturonate 59.3% alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + 38.7% galacturonate ?
3.2.1.15 additional information no activity with alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate Fusarium verticillioides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 More one major and three minor bands are reveald by SDS-PAGE, with MW of 49000 Da, 46000 Da, 42000 Da and 39000 Da, heterogeneity due to different degrees of glycosylation Fusarium verticillioides

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 PG
-
 Fusarium verticillioides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.15 additional information
-
 additional information the turnover number for homogalacturonan is 104 per sec Fusarium verticillioides
3.2.1.15 21.6
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Fusarium verticillioides
3.2.1.15 24.7
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Fusarium verticillioides
3.2.1.15 81.1
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Fusarium verticillioides
3.2.1.15 106.3
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Fusarium verticillioides
3.2.1.15 122.5
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Fusarium verticillioides