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Literature summary extracted from

  • Puchart, V.; Vrsanska, M.; Bhat, M.K.; Biely, P.
    Purification and characterization of alpha-galactosidase from a thermophilic fungus Thermomyces lanuginosus (2000), Biochim. Biophys. Acta, 1524, 27-37.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.5
-
 4-methylumbelliferyl alpha-D-galactoside 50°C, pH 4.5 Thermomyces lanuginosus
3.2.1.22 2.4
-
 melibiose 50°C, pH 4.5 Thermomyces lanuginosus
3.2.1.22 11.3
-
 raffinose 50°C, pH 4.5 Thermomyces lanuginosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.22 extracellular
-
 Thermomyces lanuginosus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 57000
-
 x * 57000, SDS-PAGE Thermomyces lanuginosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Thermomyces lanuginosus
-
 IMI 158749
-
3.2.1.22 Thermomyces lanuginosus IMI 158749
-
 IMI 158749
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.22 glycoprotein
-
 Thermomyces lanuginosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Thermomyces lanuginosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.22 culture medium
-
 Thermomyces lanuginosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 266.6
-
-
 Thermomyces lanuginosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-methylumbelliferyl alpha-D-galactoside + H2O
-
 Thermomyces lanuginosus 4-methylumbelliferone + alpha-D-galactose
-
 ?
3.2.1.22 4-methylumbelliferyl alpha-D-galactoside + H2O
-
 Thermomyces lanuginosus IMI 158749 4-methylumbelliferone + alpha-D-galactose
-
 ?
3.2.1.22 melibiose + H2O
-
 Thermomyces lanuginosus alpha-D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O
-
 Thermomyces lanuginosus IMI 158749 alpha-D-galactose + D-glucose
-
 ?
3.2.1.22 raffinose + H2O
-
 Thermomyces lanuginosus sucrose + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Thermomyces lanuginosus IMI 158749 sucrose + alpha-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 ? x * 57000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 65 70
-
 Thermomyces lanuginosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.22 50 75 50°C: about 60% of maximal activity, 75°C: about 50% of maximal activity Thermomyces lanuginosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 60
-
 pH 4.5, 50 mM sodium acetate buffer, stable for 6 h Thermomyces lanuginosus
3.2.1.22 65
-
 pH 4.5, 50 mM sodium acetate buffer, about 30% loss of activity after 2 h, about 50% loss of activitry after 3 h, about 90% loss of activity after 6 h Thermomyces lanuginosus
3.2.1.22 70
-
 pH 4.5, 50 mM sodium acetate buffer, about 75% loss of activity after 30 min Thermomyces lanuginosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.22 2.3
-
 melibiose 50°C, pH 4.5 Thermomyces lanuginosus
3.2.1.22 32.4
-
 raffinose 50°C, pH 4.5 Thermomyces lanuginosus
3.2.1.22 49.8
-
 4-methylumbelliferyl alpha-D-galactoside 50°C, pH 4.5 Thermomyces lanuginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 4.5 5
-
 Thermomyces lanuginosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 2 6 pH 2.0: about 50% of maximal activity, pH 6.0: about 50% of maximal activity Thermomyces lanuginosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 3 7.5 50°C, 5 h, stable Thermomyces lanuginosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.22 Thermomyces lanuginosus isoelectric focusing, pH-gradient 3-7
-
 4.9