Literature summary extracted from

Murata, T.; Kawano, M.; Igarashi, K.; Yamato, I.; Kakinuma, Y.
Catalytic properties of Na(+)-translocating V-ATPase in Enterococcus hirae (2001), Biochim. Biophys. Acta, 1505, 75-81.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
7.2.2.1	destruxin B	peptidic antibiotic, inhibits the ATPase activity	Enterococcus hirae
7.2.2.1	additional information	not inhibited by amiloride, vanadate or azide	Enterococcus hirae
7.2.2.1	N,N'-dicyclohexylcarbodiimide	inhibits in a pH dependent manner, binding covalently to acidic amino acid residues, inactivation is prevented by the presence of Na+ and Li+	Enterococcus hirae
7.2.2.1	N-ethylmaleimide	inhibition of ATPase activity	Enterococcus hirae
7.2.2.1	NO3-	inhibition of ATPase activity	Enterococcus hirae

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
7.2.2.1	0.02	-	Na+/in	pH 8	Enterococcus hirae
7.2.2.1	0.06	-	Li+/in	pH 8	Enterococcus hirae
7.2.2.1	3.5	-	Li+/in	pH 8	Enterococcus hirae
7.2.2.1	4	-	Na+/in	pH 8	Enterococcus hirae

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
7.2.2.1	11000	-	3 * 66000 + 3 * 51000 + 1 * 38000 + 1 * 27000 + 3 * 23000 + 2 (or 3) * 14000 + 4 (or 6) * 11000 + 1 * 76000 + 2 (or 3) * 16000, SDS-PAGE	Enterococcus hirae
7.2.2.1	14000	-	3 * 66000 + 3 * 51000 + 1 * 38000 + 1 * 27000 + 3 * 23000 + 2 (or 3) * 14000 + 4 (or 6) * 11000 + 1 * 76000 + 2 (or 3) * 16000, SDS-PAGE	Enterococcus hirae
7.2.2.1	16000	-	3 * 66000 + 3 * 51000 + 1 * 38000 + 1 * 27000 + 3 * 23000 + 2 (or 3) * 14000 + 4 (or 6) * 11000 + 1 * 76000 + 2 (or 3) * 16000, SDS-PAGE	Enterococcus hirae
7.2.2.1	23000	-	3 * 66000 + 3 * 51000 + 1 * 38000 + 1 * 27000 + 3 * 23000 + 2 (or 3) * 14000 + 4 (or 6) * 11000 + 1 * 76000 + 2 (or 3) * 16000, SDS-PAGE	Enterococcus hirae
7.2.2.1	27000	-	3 * 66000 + 3 * 51000 + 1 * 38000 + 1 * 27000 + 3 * 23000 + 2 (or 3) * 14000 + 4 (or 6) * 11000 + 1 * 76000 + 2 (or 3) * 16000, SDS-PAGE	Enterococcus hirae
7.2.2.1	38000	-	3 * 66000 + 3 * 51000 + 1 * 38000 + 1 * 27000 + 3 * 23000 + 2 (or 3) * 14000 + 4 (or 6) * 11000 + 1 * 76000 + 2 (or 3) * 16000, SDS-PAGE	Enterococcus hirae
7.2.2.1	51000	-	3 * 66000 + 3 * 51000 + 1 * 38000 + 1 * 27000 + 3 * 23000 + 2 (or 3) * 14000 + 4 (or 6) * 11000 + 1 * 76000 + 2 (or 3) * 16000, SDS-PAGE	Enterococcus hirae
7.2.2.1	66000	-	3 * 66000 + 3 * 51000 + 1 * 38000 + 1 * 27000 + 3 * 23000 + 2 (or 3) * 14000 + 4 (or 6) * 11000 + 1 * 76000 + 2 (or 3) * 16000, SDS-PAGE	Enterococcus hirae
7.2.2.1	76000	-	3 * 66000 + 3 * 51000 + 1 * 38000 + 1 * 27000 + 3 * 23000 + 2 (or 3) * 14000 + 4 (or 6) * 11000 + 1 * 76000 + 2 (or 3) * 16000, SDS-PAGE	Enterococcus hirae

Organism

EC Number	Organism	UniProt	Comment	Textmining
7.2.2.1	Enterococcus hirae	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7.2.2.1	ATP + H2O + Li+/in	coupled to Li+ translocation across the cytoplasmic membrane	Enterococcus hirae	ADP + phosphate + Li+/out	-	?
7.2.2.1	ATP + H2O + Na+/in	coupled to Na+ translocation across the cytoplasmic membrane	Enterococcus hirae	ADP + phosphate + Na+/out	-	?

Subunits

EC Number	Subunits	Comment	Organism
7.2.2.1	multimer	3 * 66000 + 3 * 51000 + 1 * 38000 + 1 * 27000 + 3 * 23000 + 2 (or 3) * 14000 + 4 (or 6) * 11000 + 1 * 76000 + 2 (or 3) * 16000, SDS-PAGE	Enterococcus hirae

Synonyms

EC Number	Synonyms	Comment	Organism
7.2.2.1	Na+-translocating ATPase	-	Enterococcus hirae
7.2.2.1	V-ATPase	-	Enterococcus hirae

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2.2.1	8	9	-	Enterococcus hirae

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
7.2.2.1	0.03	-	destruxin B	-	Enterococcus hirae
7.2.2.1	0.2	-	N-ethylmaleimide	-	Enterococcus hirae
7.2.2.1	37	-	NO3-	-	Enterococcus hirae

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Release 2023.1 (January 2023)