BRENDA - Enzyme Database show

The Na(+) cycle in Acetobacterium woodii: identification and characterization of a Na(+) translocating F(1)F(0)-ATPase with a mixed oligomer of 8 and 16 kDa proteolipids

Muller, V.; Aufurth, S.; Rahlfs, S.; Biochim. Biophys. Acta 1505, 108-120 (2001)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.2.2.1
5-(N,N-Hexamethylene)amiloride
70% inhibition at 0.1 mM, Na+ protects against inactivation
Acetobacterium woodii
7.2.2.1
5-(N-ethyl-N-isopropyl)amiloride
70% inhibition at 0.1 mM, Na+ protects against inactivation
Acetobacterium woodii
7.2.2.1
azide
inhibition of both ATPase and Na+ translocation activities
Acetobacterium woodii
7.2.2.1
diethylstilbestrol
30% inhibition at 0.001 mM
Acetobacterium woodii
7.2.2.1
additional information
not inhibited by vanadate
Acetobacterium woodii
7.2.2.1
N,N'-dicyclohexylcarbodiimide
inhibition of both ATPase and Na+ translocation activities, complete inhibition at 0.005 mM
Acetobacterium woodii
7.2.2.1
N-10-benzylamiloride
70% inhibition at 0.1 mM, Na+ protects against inactivation
Acetobacterium woodii
7.2.2.1
NO3-
50% inhibition at 10 mM
Acetobacterium woodii
7.2.2.1
tributyltin
85% inhibition at 0.001 mM
Acetobacterium woodii
7.2.2.1
Venturicidin
inhibition of both ATPase and Na+ translocation activities
Acetobacterium woodii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.2.2.1
0.4
-
ATP
-
Acetobacterium woodii
7.2.2.1
0.4
-
Na+/out
pH 7.5
Acetobacterium woodii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.2.2.1
Li+
75% of the maximal activity compared to saturating Na+ concentrations
Acetobacterium woodii
7.2.2.1
Mg2+
necessary for ATPase activity
Acetobacterium woodii
7.2.2.1
SO32-
-
Acetobacterium woodii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.2.2.1
16000
-
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
7.2.2.1
19000
-
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
7.2.2.1
35000
-
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
7.2.2.1
52000
-
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
7.2.2.1
57000
-
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
7.2.2.1
590000
-
gel filtration
Acetobacterium woodii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.2.2.1
Acetobacterium woodii
-
homoacetogenic Gram-positive bacterium
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.2.1
ADP + phosphate + Na+/out
-
654801
Acetobacterium woodii
ATP + H2O + Na+/in
-
-
-
r
7.2.2.1
CDP + phosphate + Na+/out
lower activity compared to ADP
654801
Acetobacterium woodii
CTP + H2O + Na+/in
-
-
-
r
7.2.2.1
GDP + phosphate + Na+/out
lower activity compared to ADP
654801
Acetobacterium woodii
GTP + H2O + Na+/in
-
-
-
r
7.2.2.1
IDP + phosphate + Na+/out
lower activity compared to ADP
654801
Acetobacterium woodii
ITP + H2O + Na+/in
-
-
-
r
7.2.2.1
UDP + phosphate + Na+/out
lower activity compared to ADP
654801
Acetobacterium woodii
UTP + H2O + Na+/in
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
7.2.2.1
oligomer
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2.2.1
5.5
6
at 0.1 mM of Na+
Acetobacterium woodii
7.2.2.1
7.5
9
at 5 mM of Na+
Acetobacterium woodii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.2.2.1
5-(N,N-Hexamethylene)amiloride
70% inhibition at 0.1 mM, Na+ protects against inactivation
Acetobacterium woodii
7.2.2.1
5-(N-ethyl-N-isopropyl)amiloride
70% inhibition at 0.1 mM, Na+ protects against inactivation
Acetobacterium woodii
7.2.2.1
azide
inhibition of both ATPase and Na+ translocation activities
Acetobacterium woodii
7.2.2.1
diethylstilbestrol
30% inhibition at 0.001 mM
Acetobacterium woodii
7.2.2.1
additional information
not inhibited by vanadate
Acetobacterium woodii
7.2.2.1
N,N'-dicyclohexylcarbodiimide
inhibition of both ATPase and Na+ translocation activities, complete inhibition at 0.005 mM
Acetobacterium woodii
7.2.2.1
N-10-benzylamiloride
70% inhibition at 0.1 mM, Na+ protects against inactivation
Acetobacterium woodii
7.2.2.1
NO3-
50% inhibition at 10 mM
Acetobacterium woodii
7.2.2.1
tributyltin
85% inhibition at 0.001 mM
Acetobacterium woodii
7.2.2.1
Venturicidin
inhibition of both ATPase and Na+ translocation activities
Acetobacterium woodii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.2.2.1
0.4
-
ATP
-
Acetobacterium woodii
7.2.2.1
0.4
-
Na+/out
pH 7.5
Acetobacterium woodii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.2.2.1
Li+
75% of the maximal activity compared to saturating Na+ concentrations
Acetobacterium woodii
7.2.2.1
Mg2+
necessary for ATPase activity
Acetobacterium woodii
7.2.2.1
SO32-
-
Acetobacterium woodii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.2.2.1
16000
-
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
7.2.2.1
19000
-
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
7.2.2.1
35000
-
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
7.2.2.1
52000
-
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
7.2.2.1
57000
-
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
7.2.2.1
590000
-
gel filtration
Acetobacterium woodii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.2.1
ADP + phosphate + Na+/out
-
654801
Acetobacterium woodii
ATP + H2O + Na+/in
-
-
-
r
7.2.2.1
CDP + phosphate + Na+/out
lower activity compared to ADP
654801
Acetobacterium woodii
CTP + H2O + Na+/in
-
-
-
r
7.2.2.1
GDP + phosphate + Na+/out
lower activity compared to ADP
654801
Acetobacterium woodii
GTP + H2O + Na+/in
-
-
-
r
7.2.2.1
IDP + phosphate + Na+/out
lower activity compared to ADP
654801
Acetobacterium woodii
ITP + H2O + Na+/in
-
-
-
r
7.2.2.1
UDP + phosphate + Na+/out
lower activity compared to ADP
654801
Acetobacterium woodii
UTP + H2O + Na+/in
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.2.2.1
oligomer
? * 57000 + ? * 52000 + ? * 35000 + ? * 19000 + ? * 16000 + ? * 4800, SDS-PAGE
Acetobacterium woodii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2.2.1
5.5
6
at 0.1 mM of Na+
Acetobacterium woodii
7.2.2.1
7.5
9
at 5 mM of Na+
Acetobacterium woodii