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Literature summary extracted from
- Purification and properties of exopolyphosphatase from the cytosol of Saccharomyces cerevisiae not encoded by the PPX1 gene (2004), Biochemistry, 69, 387-393.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.6.1.11 | addition of 0.05 mM CoSO4 does not increase the enzyme stability independent of the presence of Triton X-100 | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.11 | diphosphate | 1 mM, 24% activity loss; 25% inactivation at 1 mM | Saccharomyces cerevisiae |  |
| 3.6.1.11 | dithiothreitol | 0.1 mM, 13% activity loss | Saccharomyces cerevisiae | |
| 3.6.1.11 | EDTA | 0.1 mM, 100% activity loss; complete inactivation at 0.1 mM | Saccharomyces cerevisiae | |
| 3.6.1.11 | heparin | 1 mg/l 65% activity loss, 10 mg/l 95% activity loss; 95% inactivation at 0.01 mg /ml | Saccharomyces cerevisiae | |
| 3.6.1.11 | iodoacetamide | 10 mM, 26% activity loss; 25% inactivation at 10 mM | Saccharomyces cerevisiae | |
| 3.6.1.11 | additional information | not inhibited by antibodies suppressing the activity of 40 kDa-exopolyphosphatase | Saccharomyces cerevisiae | |
| 3.6.1.11 | NaF | 10 mM, 9% activity loss | Saccharomyces cerevisiae | |
| 3.6.1.11 | polyphosphate 3 | 1mM, 4% activity loss | Saccharomyces cerevisiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.11 | 0.0035 | - |
(Polyphosphate)n | n = 208, pH 7.2, 30°C | Saccharomyces cerevisiae | |
| 3.6.1.11 | 0.075 | - |
(Polyphosphate)n | n = 15, pH 7.2, 30°C | Saccharomyces cerevisiae | |
| 3.6.1.11 | 1.1 | - |
(Polyphosphate)n | n = 3, pH 7.2, 30°C | Saccharomyces cerevisiae | |
| 3.6.1.11 | 3.5 | - |
polyphosphate 208 | - |
Saccharomyces cerevisiae | |
| 3.6.1.11 | 75 | - |
polyphosphate 15 | - |
Saccharomyces cerevisiae | |
| 3.6.1.11 | 1100 | - |
polyphosphate 3 | - |
Saccharomyces cerevisiae |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.6.1.11 | cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.11 | Co2+ | 6fold activation at 0.1 mM | Saccharomyces cerevisiae | |
| 3.6.1.11 | Co2+ | 0.1 mM, 6fold activity stimulation | Saccharomyces cerevisiae | |
| 3.6.1.11 | KCl | 50 and 200 mM, 39 and 38% activity enhancement, respectively | Saccharomyces cerevisiae | |
| 3.6.1.11 | Mg2+ | 2fold activation at 0.1 mM | Saccharomyces cerevisiae | |
| 3.6.1.11 | Mg2+ | 0.1 mM MgSO4, 5% activity loss | Saccharomyces cerevisiae | |
| 3.6.1.11 | Mg2+ | 1 mM, 2fold activity enhancement | Saccharomyces cerevisiae | |
| 3.6.1.11 | additional information | stimulated by divalent cations to a lesser extent | Saccharomyces cerevisiae | |
| 3.6.1.11 | NaCl | 50 and 200 mM, 46 and 42% activity enhancement, respectively | Saccharomyces cerevisiae | |
| 3.6.1.11 | NH4Cl | 50 and 200 mM, 42 and 67% activity enhancement, respectively | Saccharomyces cerevisiae | |
| 3.6.1.11 | Zn2+ | 1.5fold activation at 0.1 mM | Saccharomyces cerevisiae | |
| 3.6.1.11 | Zn2+ | 0.1 mM ZnSO4, 76% activity loss | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.11 | 32000 | - |
x * 32000 + x * 35000, polyphosphate seems to stabilize the complex | Saccharomyces cerevisiae |
| 3.6.1.11 | 35000 | - |
x * 32000 + x * 35000, polyphosphate seems to stabilize the complex | Saccharomyces cerevisiae |
| 3.6.1.11 | 125000 | - |
Superose 6 column gel filtration, active but unstable enzyme complex | Saccharomyces cerevisiae |
| 3.6.1.11 | 500000 | - |
Superose 6 column gel filtration, stable enzyme complex composed of two polypeptides of 32 and 35 kDa and apparently polyphosphates | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.11 | Saccharomyces cerevisiae | - |
- |
- |
| 3.6.1.11 | Saccharomyces cerevisiae VKM Y-1173 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.11 | DEAE-Toyopearl 650 M column chromatography | Saccharomyces cerevisiae |
| 3.6.1.11 | partial, using ion-exchange chromatography and gel filtration | Saccharomyces cerevisiae |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.6.1.11 | (polyphosphate)n + H2O = (polyphosphate)n-1 + phosphate | hydrolyzes poly-phosphates with an average chain length of 208 to 15 phosphate residues | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.11 | additional information | - |
no specific activity on diphosphate, ATP and p-nitrophenyl phosphate | Saccharomyces cerevisiae |
| 3.6.1.11 | 0.47 | - |
enzyme from cystosol | Saccharomyces cerevisiae |
| 3.6.1.11 | 1.11 | - |
after ammonium sulfate precipitation | Saccharomyces cerevisiae |
| 3.6.1.11 | 5 | - |
after DEAE-Toyopearl 650 M column chromatography | Saccharomyces cerevisiae |
| 3.6.1.11 | 150 | - |
pH 7.2, 30°C, polyP208 as substrate | Saccharomyces cerevisiae |
| 3.6.1.11 | 150 | - |
after 319fold purification with heparin agarose, at 30°C in 1 ml of reaction mixture containing 50 mM Tris-HCl buffer, pH 7.2, 0.1 mM CoSO4, 200 mM ammonium chloride, and 0.01 mM polyphosphate 208 | Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.6.1.11 | -4°C, 0.1% Triton X-100, 1M KCl, 4 days, no activity loss | Saccharomyces cerevisiae |
| 3.6.1.11 | -4°C, enzyme desorbed from heparin-agarose with 1 M KCl and 0.1% Triton X-100, 4 days, no loss of activity | Saccharomyces cerevisiae |
| 3.6.1.11 | 4°C, enzyme obtained by desorption with polyphosphate, 2 h, 30% loss of activity | Saccharomyces cerevisiae |
| 3.6.1.11 | 92°C, in the presence of 0.1% Triton X-100, 5 days, 8% loss of activity of the partially purified exopolyphosphatase preparation after DEAE-Toyopearl chromatography | Saccharomyces cerevisiae |
| 3.6.1.11 | 92°C, without 0.1% Triton X-100, 5 days, 66% loss of activity of the partially purified exopolyphosphatase preparation after DEAE-Toyopearl chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.11 | (polyphosphate)n + H2O | - |
Saccharomyces cerevisiae | (polyphosphate)n-1 + phosphate | - |
? | |
| 3.6.1.11 | (polyphosphate)n + H2O | - |
Saccharomyces cerevisiae VKM Y-1173 | (polyphosphate)n-1 + phosphate | - |
? | |
| 3.6.1.11 | additional information | ATP, diphosphate and p-nitrophenyl phosphate are not substrates | Saccharomyces cerevisiae | ? | - |
? | |
| 3.6.1.11 | additional information | ATP, diphosphate and p-nitrophenyl phosphate are not substrates | Saccharomyces cerevisiae VKM Y-1173 | ? | - |
? | |
| 3.6.1.11 | polyphosphate 15 + H2O | - |
Saccharomyces cerevisiae | polyphosphate 14 + phosphate | - |
? | |
| 3.6.1.11 | polyphosphate 15 + H2O | - |
Saccharomyces cerevisiae VKM Y-1173 | polyphosphate 14 + phosphate | - |
? | |
| 3.6.1.11 | polyphosphate 208 + H2O | - |
Saccharomyces cerevisiae | polyphosphate 207 + phosphate | - |
? | |
| 3.6.1.11 | polyphosphate 208 + H2O | - |
Saccharomyces cerevisiae VKM Y-1173 | polyphosphate 207 + phosphate | - |
? | |
| 3.6.1.11 | polyphosphate 3 + H2O | - |
Saccharomyces cerevisiae | polyphosphate 2 + phosphate | - |
? | |
| 3.6.1.11 | polyphosphate 3 + H2O | - |
Saccharomyces cerevisiae VKM Y-1173 | polyphosphate 2 + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.1.11 | ? | x * 32000 + x * 35000, polyphosphate seems to stabilize the complex | Saccharomyces cerevisiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.6.1.11 | high molecular mass exopolyphosphatase | - |
Saccharomyces cerevisiae |
| 3.6.1.11 | polyphosphate phosphohydrolase | - |
Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.11 | 7 | - |
- |
Saccharomyces cerevisiae |
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