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Literature summary extracted from

  • Heredia, V.V.; Cooper, W.C.; Kruger, R.G.; Jin, Y.; Penning, T.M.
    Alanine scanning mutagenesis of the testosterone binding site of rat 3alpha-hydroxysteroid dehydrogenase demonstrates contact residues influence the rate-determining step (2004), Biochemistry, 43, 5832-5841.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.213 overexpression of wild-type and mutant enzymes in Escherichia coli C41(DE3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.213 F118A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview Rattus norvegicus
1.1.1.213 F129A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview Rattus norvegicus
1.1.1.213 L54A site-directed mutagenesis, reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview Rattus norvegicus
1.1.1.213 N306A site-directed mutagenesis, reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview Rattus norvegicus
1.1.1.213 T226A site-directed mutagenesis, reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview Rattus norvegicus
1.1.1.213 T24A site-directed mutagenesis, reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview Rattus norvegicus
1.1.1.213 W227A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, 23fold decreased affinity for progesterone, effects of mutation on binding constants and kinetics, overview Rattus norvegicus
1.1.1.213 Y310A site-directed mutagenesis, reduced activity compared to the wild-type enzyme, effects of mutation on binding constants and kinetics, overview Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.213 progesterone competitive to testosterone Rattus norvegicus
1.1.1.213 testosterone competitive to progesterone Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.213 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.213 recombinant wild-type and mutant enzymes from Escherichia coli C41(DE3), to homogeneity Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.213 a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ ordered bi bi reaction mechanism Rattus norvegicus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.213 0.006
-
 purified recombinant mutant W227A, substrate androsterone Rattus norvegicus
1.1.1.213 0.01
-
 purified recombinant mutant F118A, substrate androsterone Rattus norvegicus
1.1.1.213 0.03
-
 purified recombinant mutant F129A, substrate androsterone Rattus norvegicus
1.1.1.213 0.08
-
 purified recombinant mutant L54A, substrate androsterone Rattus norvegicus
1.1.1.213 0.09
-
 purified recombinant mutant N306A, substrate androsterone Rattus norvegicus
1.1.1.213 0.1
-
 purified recombinant mutant Y310A, substrate androsterone Rattus norvegicus
1.1.1.213 0.86
-
 purified recombinant mutant T24A, substrate androsterone Rattus norvegicus
1.1.1.213 0.96
-
 purified recombinant mutant T226A, substrate androsterone Rattus norvegicus
1.1.1.213 1.6
-
 purified recombinant wild-type enzyme, substrate androsterone Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.213 5beta-androstan-3alpha-ol-17-one + NAD(P)+
-
 Rattus norvegicus 5beta-androstane-3,17-dione + NAD(P)H
-
 r
1.1.1.213 5beta-pregnan-3alpha-ol-20-one + NAD(P)+
-
 Rattus norvegicus 5beta-pregnane-3,20-dione + NAD(P)H
-
 r
1.1.1.213 androsterone + NAD(P)+
-
 Rattus norvegicus 5alpha-androstane-3,17-dione + NAD(P)H
-
 r
1.1.1.213 progesterone + NAD(P)H
-
 Rattus norvegicus 3alpha-hydroxy-pregn-4-ene-20-one + NAD(P)+
-
 r
1.1.1.213 testosterone + NAD(P)H + H+
-
 Rattus norvegicus 4-androsten-3alpha,17beta-diol + NAD(P)+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.213 3alpha-HSD
-
 Rattus norvegicus
1.1.1.213 3alpha-hydroxysteroid dehydrogenase
-
 Rattus norvegicus
1.1.1.213 AKR1C9
-
 Rattus norvegicus
1.1.1.213 More see also EC 1.1.1.50, 3alpha-hydroxysteroid dehydrogenase (B-specific), for enzymes catalyzing the same reaction, but which are B-specific with respect to hydrogen transfer to/from NAD(P)+/NAD(P)H. Enzymes without information of stereospecificity are summarized under EC 1.1.1.50, but may in fact belong to EC 1.1.1.213 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.213 additional information
-
 additional information kcat in steady-state, single turnover, burst in multiple turnover, steady-state in multiple turnover Rattus norvegicus
1.1.1.213 0.0053
-
 androsterone steady-state, pH 8.0, 25°C, recombinant mutant W227A Rattus norvegicus
1.1.1.213 0.0083
-
 androsterone steady-state, pH 8.0, 25°C, recombinant mutant F118A Rattus norvegicus
1.1.1.213 0.57
-
 androsterone steady-state, pH 8.0, 25°C, recombinant mutant T226A Rattus norvegicus
1.1.1.213 0.83
-
 androsterone steady-state, pH 8.0, 25°C, recombinant wild-type enzyme Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.213 7
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.213 NAD(P)+ A-specific Rattus norvegicus
1.1.1.213 NAD(P)H A-specific Rattus norvegicus