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Literature summary extracted from

  • Su, P.; Scheiner-Bobis, G.
    Lys691 and Asp714 of the Na+/K+-ATPase a subunit are essential for phosphorylation, dephosphorylation, and enzyme turnover (2004), Biochemistry, 43, 4731-4740.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.13 expression of subunit alpha 1 in Saccharomyces cerevisiae together with dog subunit beta1 Ovis aries

Protein Variants

EC Number Protein Variants Comment Organism
7.2.2.13 D714A insensitive to palytoxin Ovis aries
7.2.2.13 D714E the IC50 of the palytoxin-induced K+ efflux is 4fold higher than in cells expressing the wild-type enzyme Ovis aries
7.2.2.13 D714R insensitive to palytoxin Ovis aries
7.2.2.13 K691A insensitive to palytoxin Ovis aries
7.2.2.13 K691D insensitive to palytoxin Ovis aries
7.2.2.13 K691R the EC50 of the palytoxin-induced K+ efflux is 7fold higher than in cells expressing the wild-type enzyme Ovis aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.13 palytoxin
-
 Ovis aries

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.13 Mg2+
-
 Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.13 Ovis aries
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.2.2.13 kidney
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2.2.13 120
-
 mutant K691A Ovis aries
7.2.2.13 175
-
 mutant D714A Ovis aries
7.2.2.13 195
-
 mutant K691R Ovis aries
7.2.2.13 575
-
 mutant D714E Ovis aries
7.2.2.13 1105
-
 wild-type Ovis aries