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Literature summary extracted from

  • Argyrou, A.; Blanchard, J.S.
    Kinetic and chemical mechanism of Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate isomeroreductase (2004), Biochemistry, 43, 4375-4384.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267
-
Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.267 additional information removal of C-terminal 18 amino acid residues has no effect on enzyme activity, affinity for substrates or sensitivity to isotopic probes Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 0.0004
-
NADPH presence of Co2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.0033
-
NADPH presence of Mn2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.004
-
1-deoxy-D-xylulose-5-phosphate cosubstrate NADPH, presence of Co2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.005
-
NADPH presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.042
-
1-deoxy-D-xylulose-5-phosphate cosubstrate NADPH, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.042
-
methyl-D-erythritol-4-phosphate presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.05
-
1-deoxy-D-xylulose-5-phosphate cosubstrate NADH, presence of Co2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.05
-
NADH presence of Co2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.1
-
1-deoxy-D-xylulose-5-phosphate cosubstrate NADPH, presence of Mn2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.17
-
NADP+ presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.21
-
1-deoxy-D-xylulose-5-phosphate cosubstrate NADH, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.24
-
1-deoxy-D-xylulose-5-phosphate cosubstrate NADH, presence of Mn2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.26
-
NADH presence of Mn2+, pH 7.5, 25°C Mycobacterium tuberculosis
1.1.1.267 0.41
-
NADH presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Co2+ required, Km-value 0.0012 mM Mycobacterium tuberculosis
1.1.1.267 Mg2+ can partly substitute for Co2+, Km-value 1.2 mM Mycobacterium tuberculosis
1.1.1.267 Mn2+ can poorly replace for Co2+, Km-value 2.4 mM Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Mycobacterium tuberculosis
-
recombinant enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.267 recombinant enzyme Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.267 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ steady-state random mechanism Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
1.1.1.267 -20°C, 50% glycerol, fully active for over 12 months Mycobacterium tuberculosis
1.1.1.267 4°C, half-life about 3 days Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose-5-phosphate + NADH
-
Mycobacterium tuberculosis methyl-D-erythritol-4-phosphate + NAD+
-
r
1.1.1.267 1-deoxy-D-xylulose-5-phosphate + NADPH
-
Mycobacterium tuberculosis methyl-D-erythritol-4-phosphate + NADP+
-
r
1.1.1.267 methyl-D-erythritol-4-phosphate + NADP+
-
Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate + NADPH
-
r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NAD+
-
Mycobacterium tuberculosis
1.1.1.267 NADH
-
Mycobacterium tuberculosis
1.1.1.267 NADP+
-
Mycobacterium tuberculosis
1.1.1.267 NADPH
-
Mycobacterium tuberculosis