EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.267 | - |
Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.267 | additional information | removal of C-terminal 18 amino acid residues has no effect on enzyme activity, affinity for substrates or sensitivity to isotopic probes | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.267 | 0.0004 | - |
NADPH | presence of Co2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.0033 | - |
NADPH | presence of Mn2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.004 | - |
1-deoxy-D-xylulose-5-phosphate | cosubstrate NADPH, presence of Co2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.005 | - |
NADPH | presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.042 | - |
1-deoxy-D-xylulose-5-phosphate | cosubstrate NADPH, presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.042 | - |
methyl-D-erythritol-4-phosphate | presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.05 | - |
1-deoxy-D-xylulose-5-phosphate | cosubstrate NADH, presence of Co2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.05 | - |
NADH | presence of Co2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.1 | - |
1-deoxy-D-xylulose-5-phosphate | cosubstrate NADPH, presence of Mn2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.17 | - |
NADP+ | presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.21 | - |
1-deoxy-D-xylulose-5-phosphate | cosubstrate NADH, presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.24 | - |
1-deoxy-D-xylulose-5-phosphate | cosubstrate NADH, presence of Mn2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.26 | - |
NADH | presence of Mn2+, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.1.1.267 | 0.41 | - |
NADH | presence of Mg2+, pH 7.5, 25°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.267 | Co2+ | required, Km-value 0.0012 mM | Mycobacterium tuberculosis | |
1.1.1.267 | Mg2+ | can partly substitute for Co2+, Km-value 1.2 mM | Mycobacterium tuberculosis | |
1.1.1.267 | Mn2+ | can poorly replace for Co2+, Km-value 2.4 mM | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.267 | Mycobacterium tuberculosis | - |
recombinant enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.267 | recombinant enzyme | Mycobacterium tuberculosis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.267 | 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | steady-state random mechanism | Mycobacterium tuberculosis |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.267 | -20°C, 50% glycerol, fully active for over 12 months | Mycobacterium tuberculosis |
1.1.1.267 | 4°C, half-life about 3 days | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.267 | 1-deoxy-D-xylulose-5-phosphate + NADH | - |
Mycobacterium tuberculosis | methyl-D-erythritol-4-phosphate + NAD+ | - |
r | |
1.1.1.267 | 1-deoxy-D-xylulose-5-phosphate + NADPH | - |
Mycobacterium tuberculosis | methyl-D-erythritol-4-phosphate + NADP+ | - |
r | |
1.1.1.267 | methyl-D-erythritol-4-phosphate + NADP+ | - |
Mycobacterium tuberculosis | 1-deoxy-D-xylulose-5-phosphate + NADPH | - |
r |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.267 | NAD+ | - |
Mycobacterium tuberculosis | |
1.1.1.267 | NADH | - |
Mycobacterium tuberculosis | |
1.1.1.267 | NADP+ | - |
Mycobacterium tuberculosis | |
1.1.1.267 | NADPH | - |
Mycobacterium tuberculosis |