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Literature summary extracted from

  • Nieman, C.E.; Wong, A.W.; He, S.; Clarke, L.; Hopwood, J.J.; Withers, S.G.
    Family 39 alpha-L-iduronidases and beta-D-xylosidases react through similar glycosyl-enzyme intermediates: Identification of the human iduronidase nucleophile (2003), Biochemistry, 42, 8054-8065.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.76 2-fluoro-alpha-L-idopyranosyluronic acid fluoride reversible, competitive with 4-nitrophenyl-alpha-L-idopyranosiduronate, activity drops down immediately after inhibitor is added Homo sapiens
3.2.1.76 5-fluoro-alpha-L-idopyranosyluronic acid fluoride reversible, competitive with 4-methylumbelliferyl-alpha-L-iduronide, activity drops down immediately after inhibitor is added Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.76 0.015
-
 4-methylumbelliferyl-alpha-L-iduronide pH 4.5, 37°C Homo sapiens
3.2.1.76 0.53
-
 4-nitrophenyl-alpha-L-idopyranosiduronate pH 4.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.76 additional information Homo sapiens cleaves alpha-L-iduronic acid from the nonreducing ends of dermatan sulfate, heparin, and heparan sulfate, deficiency in alpha-L-iduronidase causes mucopolysaccharidosis type I ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.76 Methanol quite stable towards molar concentrations of methanol Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.76 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.76 4-methylumbelliferyl-alpha-L-iduronide + H2O
-
 Homo sapiens 4-methylumbelliferone + alpha-L-iduronic acid
-
 ?
3.2.1.76 4-methylumbelliferyl-alpha-L-iduronide + methanol methanolysis Homo sapiens ?
-
 ?
3.2.1.76 4-nitrophenyl-alpha-L-idopyranosiduronate + H2O
-
 Homo sapiens 4-nitrophenol + alpha-L-iduronic acid
-
 ?
3.2.1.76 5-fluoro-alpha-L-idopyranosyluronic acid fluoride + H2O
-
 Homo sapiens fluoride + ?
-
 ?
3.2.1.76 additional information cleaves alpha-L-iduronic acid from the nonreducing ends of dermatan sulfate, heparin, and heparan sulfate, deficiency in alpha-L-iduronidase causes mucopolysaccharidosis type I Homo sapiens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.76 0.83
-
 5-fluoro-alpha-L-idopyranosyluronic acid fluoride pH 4.5, 37°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.76 0.0012
-
 5-fluoro-alpha-L-idopyranosyluronic acid fluoride pH 4.5, 37°C Homo sapiens
3.2.1.76 0.0046
-
 2-fluoro-alpha-L-idopyranosyluronic acid fluoride pH 4.5, 37°C Homo sapiens