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Literature summary extracted from
- Steady-state and pre-steady-state kinetic analysis of halopropane conversion by a Rhodococcus haloalkane dehalogenase (2003), Biochemistry, 42, 8047-8053.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | expression in Escherichia coli | Rhodococcus rhodochrous |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.5 | additional information | - |
additional information | steady-state and pre-steady-state kintic analysis, kinetic mechanism of dehalogenation | Rhodococcus rhodochrous | |
| 3.8.1.5 | 0.005 | - |
1,3-dibromopropane | pH 9.4, 30°C | Rhodococcus rhodochrous |  |
| 3.8.1.5 | 0.3 | - |
1,2,3-tribromopropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 0.6 | - |
1-Bromopropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 0.63 | - |
1,3-Dichloropropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 1 | - |
1,2-dibromopropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 1 | - |
1-Chloropropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 2.2 | - |
1,2,3-trichloropropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 3.3 | - |
3-bromo-1-propanol | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 4 | - |
1,2-Dibromoethane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 11 | - |
2-bromoethanol | above, pH 9.4, 30°C | Rhodococcus rhodochrous | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.8.1.5 | Rhodococcus rhodochrous | - |
enzyme DhaA | - |
| 3.8.1.5 | Rhodococcus rhodochrous NCIMB 13064 | - |
enzyme DhaA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | recombinant enzyme from Escherichia coli | Rhodococcus rhodochrous |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | additional information | - |
- |
Rhodococcus rhodochrous |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.5 | 1,2,3-tribromopropane + H2O | - |
Rhodococcus rhodochrous | 2,3-dibromo-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1,2,3-tribromopropane + H2O | - |
Rhodococcus rhodochrous NCIMB 13064 | 2,3-dibromo-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1,2,3-trichloropropane + H2O | low activity | Rhodococcus rhodochrous | 2,3-dichloro-1-propanol + chloride | - |
? | |
| 3.8.1.5 | 1,2,3-trichloropropane + H2O | low activity | Rhodococcus rhodochrous NCIMB 13064 | 2,3-dichloro-1-propanol + chloride | - |
? | |
| 3.8.1.5 | 1,2-dibromoethane + H2O | - |
Rhodococcus rhodochrous | 2-bromo-1-ethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromopropane + H2O | - |
Rhodococcus rhodochrous | 2-bromo-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dichloropropane + H2O | - |
Rhodococcus rhodochrous | 2-chloro-1-propanol + chloride | - |
? | |
| 3.8.1.5 | 1,3-dibromopropane + H2O | best substrate, the release of product 3-bromo-1-propanol is the rate limiting step | Rhodococcus rhodochrous | 3-bromo-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1,3-dichloropropane + H2O | - |
Rhodococcus rhodochrous | 3-chloro-1-propanol + chloride | - |
? | |
| 3.8.1.5 | 1-bromopropane + H2O | - |
Rhodococcus rhodochrous | propanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromopropane + H2O | - |
Rhodococcus rhodochrous NCIMB 13064 | propanol + bromide | - |
? | |
| 3.8.1.5 | 1-chloropropane + H2O | - |
Rhodococcus rhodochrous | propanol + chloride | - |
? | |
| 3.8.1.5 | 1-chloropropane + H2O | - |
Rhodococcus rhodochrous NCIMB 13064 | propanol + chloride | - |
? | |
| 3.8.1.5 | 2-bromoethanol + H2O | low activity | Rhodococcus rhodochrous | ethanol + bromide | - |
? | |
| 3.8.1.5 | 2-bromoethanol + H2O | low activity | Rhodococcus rhodochrous NCIMB 13064 | ethanol + bromide | - |
? | |
| 3.8.1.5 | 3-bromo-1-propanol + H2O | - |
Rhodococcus rhodochrous | ethanol + bromide | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | DhaA | - |
Rhodococcus rhodochrous |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 30 | - |
assay at | Rhodococcus rhodochrous |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.5 | additional information | - |
additional information | rates of reaction steps | Rhodococcus rhodochrous | |
| 3.8.1.5 | 0.08 | - |
1,2,3-trichloropropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 0.48 | - |
1-Chloropropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 1.3 | - |
1,3-Dichloropropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 2.3 | - |
1,2-dibromopropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 2.7 | - |
1,3-dibromopropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 2.7 | - |
1-Bromopropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 3 | - |
2-bromoethanol | above, pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 3 | - |
3-bromo-1-propanol | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 3.6 | - |
1,2,3-tribromopropane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
| 3.8.1.5 | 14.3 | - |
1,2-Dibromoethane | pH 9.4, 30°C | Rhodococcus rhodochrous | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 9.4 | - |
assay at | Rhodococcus rhodochrous |
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