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Literature summary extracted from

  • Lapshina, E.A.; Belyaeva, O.V.; Chumakova, O.V.; Kedishvili, N.Y.
    Differential recognition of the free versus bound retinol by human microsomal retinol/sterol dehydrogenases: characterization of the holo-CRBP dehydrogenase activity of RoDH-4 (2003), Biochemistry, 42, 776-784.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.105 cellular retinol binding protein free form without retinol Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.105 0.0002
-
 androsterone pH 7.3, 37°C Homo sapiens
1.1.1.105 0.00024
-
 NAD+ pH 7.3, 37°C Homo sapiens
1.1.1.105 0.0027
-
 retinol bound to cellular retinol binding protein pH 7.3, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.105 microsome integral membrane protein facing cytosolic side of the membrane Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.105 36000
-
 x * 36000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.105 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.105 no glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.105 solubilized enzyme is extremely unstable and loses almost 80% of the initial activity even at partially purified stage, purification uses resistance of enzyme to proteolysis at 4°C in the presence of 1% Triton Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.1.1.105 4°C, reconstitution of enzyme into proteoliposomes, stable for at least 4 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.105 androsterone + NAD+
-
 Homo sapiens ? + NADH
-
 ?
1.1.1.105 additional information substrate bound to cellular retinol binding protein is preferred Homo sapiens ?
-
 ?
1.1.1.105 retinol + NAD+
-
 Homo sapiens retinal + NADH
-
 ?
1.1.1.105 retinol bound to cellular retinol binding protein + NAD+
-
 Homo sapiens retinal bound to cellular retinol binding protein + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.105 ? x * 36000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.105 RoDH-4
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.105 NAD+
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.105 0.00003
-
 cellular retinol binding protein pH 7.3, 37°C Homo sapiens