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Literature summary extracted from

  • Sivaraman, S.; Zwahlen, J.; Bell, A.F.; Hedstrom, L.; Tonge, P.J.
    Structure-activity studies of the inhibition of FabI, the enoyl reductase from Escherichia coli, by triclosan: kinetic analysis of mutant FabIs (2003), Biochemistry, 42, 4406-4413.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.9 A197M no effect on triclosan inhibition Escherichia coli
1.3.1.9 F203L mutation decreases affinity of triclosan Escherichia coli
1.3.1.9 G93V mutation decreases affinity of triclosan Escherichia coli
1.3.1.9 M159T mutation decreases affinity of triclosan Escherichia coli
1.3.1.9 Y156F mutation decreases affinity of triclosan Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 2,4,4'-trichloro-2'-hydroxydiphenyl ether trivial name triclosan Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.9 0.003
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, G93V mutant Escherichia coli
1.3.1.9 0.009
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, A197M mutant Escherichia coli
1.3.1.9 0.01
-
 NADH 25°C, pH 8.0, G93V mutant Escherichia coli
1.3.1.9 0.02
-
 NADH 25°C, pH 8.0 Escherichia coli
1.3.1.9 0.029
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, Y156F mutant Escherichia coli
1.3.1.9 0.03
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0 Escherichia coli
1.3.1.9 0.035
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, F203L mutant Escherichia coli
1.3.1.9 0.037
-
 NADH 25°C, pH 8.0, F203L mutant Escherichia coli
1.3.1.9 0.051
-
 NADH 25°C, pH 8.0, Y156F mutant Escherichia coli
1.3.1.9 0.079
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, M159T mutant Escherichia coli
1.3.1.9 0.35
-
 NADH 25°C, pH 8.0, A197M mutant Escherichia coli
1.3.1.9 0.4
-
 NADH 25°C, pH 8.0, M159T mutant Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Escherichia coli P0AEK4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 trans-2-dodecenoyl-CoA + NADH
-
 Escherichia coli dodecanoyl-CoA + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 FabI
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.9 0.45
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, M159T mutant Escherichia coli
1.3.1.9 1.75
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, Y156F mutant Escherichia coli
1.3.1.9 5
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, G93V mutant Escherichia coli
1.3.1.9 8.15
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, A197M mutant Escherichia coli
1.3.1.9 10.6
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0, F203L mutant Escherichia coli
1.3.1.9 11.9
-
 trans-2-dodecenoyl-CoA 25°C, pH 8.0 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NADH
-
 Escherichia coli