BRENDA - Enzyme Database

Spectrophotometric and steady-state kinetic analysis of the biosynthetic arginine decarboxylase of Yersinia pestis utilizing arginine analogues as inhibitors and alternative substrates

Balbo, P.B.; Patel, C.N.; Sell, K.G.; Adcock, R.S.; Neelakantan, S.; Crooks, P.A.; Oliveira, M.A.; Biochemistry 42, 15189-15196 (2003)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
agmatine
-
Yersinia pestis
4.1.1.19
D-Arg
-
Yersinia pestis
4.1.1.19
guanidine
-
Yersinia pestis
4.1.1.19
L-arginine methyl ester
-
Yersinia pestis
4.1.1.19
L-arginineamide
-
Yersinia pestis
4.1.1.19
L-argininic acid
-
Yersinia pestis
4.1.1.19
L-canavanine
-
Yersinia pestis
4.1.1.19
L-homoarginine
-
Yersinia pestis
4.1.1.19
methylguanidine
-
Yersinia pestis
4.1.1.19
N-benzylguanidine
-
Yersinia pestis
4.1.1.19
n-propylguanidine
-
Yersinia pestis
4.1.1.19
Ngamma-methyl-D-arginine
-
Yersinia pestis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
0.163
-
Ngamma-methyl-L-arginine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.2
-
L-Arg
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.233
-
L-canavanine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
9.53
-
L-N5-(iminoethyl)-ornithine
pH 7.5, 37°C
Yersinia pestis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.19
Yersinia pestis
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-arginine
-
654689
Yersinia pestis
agmatine + CO2
-
-
-
?
4.1.1.19
L-canavanine
-
654689
Yersinia pestis
N-(3-aminopropoxy)guanidine + CO2
-
-
-
?
4.1.1.19
L-N5-(iminoethyl)-ornithine
-
654689
Yersinia pestis
N1-(2-iminoethyl)-butane-1,4-diamine + CO2
-
-
-
?
4.1.1.19
Ngamma-monomethyl-L-Arg
-
654689
Yersinia pestis
N-(3-aminopropyl)-N'-methylguanidine + CO2
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.19
0.11
-
L-canavanine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.419
-
L-N5-(iminoethyl)-ornithine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
1.22
-
Ngamma-methyl-L-arginine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
5.46
-
L-Arg
pH 7.5, 37°C
Yersinia pestis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.1.19
0.0206
-
D-Arg
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.168
-
NG-methyl-D-arginine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.189
-
L-arginine methyl ester
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.327
-
L-argininic acid
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.427
-
L-canavanine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.897
-
L-argininamide
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
1.4
-
N-benzylguanidine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
1.53
-
L-homoarginine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
1.93
-
n-propylguanidine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
2.77
-
agmatine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
3.09
-
methylguanidine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
3.39
-
guanidine
pH 7.5, 37°C
Yersinia pestis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
agmatine
-
Yersinia pestis
4.1.1.19
D-Arg
-
Yersinia pestis
4.1.1.19
guanidine
-
Yersinia pestis
4.1.1.19
L-arginine methyl ester
-
Yersinia pestis
4.1.1.19
L-arginineamide
-
Yersinia pestis
4.1.1.19
L-argininic acid
-
Yersinia pestis
4.1.1.19
L-canavanine
-
Yersinia pestis
4.1.1.19
L-homoarginine
-
Yersinia pestis
4.1.1.19
methylguanidine
-
Yersinia pestis
4.1.1.19
N-benzylguanidine
-
Yersinia pestis
4.1.1.19
n-propylguanidine
-
Yersinia pestis
4.1.1.19
Ngamma-methyl-D-arginine
-
Yersinia pestis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.1.19
0.0206
-
D-Arg
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.168
-
NG-methyl-D-arginine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.189
-
L-arginine methyl ester
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.327
-
L-argininic acid
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.427
-
L-canavanine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.897
-
L-argininamide
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
1.4
-
N-benzylguanidine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
1.53
-
L-homoarginine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
1.93
-
n-propylguanidine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
2.77
-
agmatine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
3.09
-
methylguanidine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
3.39
-
guanidine
pH 7.5, 37°C
Yersinia pestis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
0.163
-
Ngamma-methyl-L-arginine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.2
-
L-Arg
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.233
-
L-canavanine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
9.53
-
L-N5-(iminoethyl)-ornithine
pH 7.5, 37°C
Yersinia pestis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-arginine
-
654689
Yersinia pestis
agmatine + CO2
-
-
-
?
4.1.1.19
L-canavanine
-
654689
Yersinia pestis
N-(3-aminopropoxy)guanidine + CO2
-
-
-
?
4.1.1.19
L-N5-(iminoethyl)-ornithine
-
654689
Yersinia pestis
N1-(2-iminoethyl)-butane-1,4-diamine + CO2
-
-
-
?
4.1.1.19
Ngamma-monomethyl-L-Arg
-
654689
Yersinia pestis
N-(3-aminopropyl)-N'-methylguanidine + CO2
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.19
0.11
-
L-canavanine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
0.419
-
L-N5-(iminoethyl)-ornithine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
1.22
-
Ngamma-methyl-L-arginine
pH 7.5, 37°C
Yersinia pestis
4.1.1.19
5.46
-
L-Arg
pH 7.5, 37°C
Yersinia pestis