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Literature summary extracted from

  • Wu, J.; Bell, A.F.; Luo, L.; Stephens, A.W.; Stankovich, M.T.; Tonge, P.J.
    Probing hydrogen-bonding interactions in the active site of medium-chain acyl-CoA dehydrogenase using Raman spectroscopy (2003), Biochemistry, 42, 11846-11856.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.7 E276Q no detectable activity in ferricenium assay Sus scrofa
1.3.8.7 E376P no detectable activity in ferricenium assay Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.7 0.0022
-
 octanoyl-CoA 25°C, with ferricenium as acceptor, pig enzyme reconstituted with 2'-deoxy-FAD Sus scrofa
1.3.8.7 0.0023
-
 octanoyl-CoA 25°C, with ferricenium as acceptor, native enzyme Sus scrofa
1.3.8.7 0.0025
-
 octanoyl-CoA 25°C, with ferricenium as acceptor, recombinant wild-type enzyme Sus scrofa
1.3.8.7 0.0026
-
 octanoyl-CoA 25°C, with ferricenium as acceptor, pig enzyme reconstituted with commercial FAD Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.7 Sus scrofa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.7 octanoyl-CoA + ferricenium
-
 Sus scrofa 2-octenoyl-CoA + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.8.7 medium-chain acyl-CoA dehydrogenase
-
 Sus scrofa
1.3.8.7 pMCAD
-
 Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.7 0.047
-
 octanoyl-CoA 25°C, with ferricenium as acceptor, pig enzyme reconstituted with 2'-deoxy-FAD Sus scrofa
1.3.8.7 18.8
-
 octanoyl-CoA 25°C, with ferricenium as acceptor, recombinant wild-type enzyme Sus scrofa
1.3.8.7 19.6
-
 octanoyl-CoA 25°C, with ferricenium as acceptor, native enzyme Sus scrofa
1.3.8.7 20
-
 octanoyl-CoA 25°C, with ferricenium as acceptor, pig enzyme reconstituted with commercial FAD Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.7 FAD
-
 Sus scrofa