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Literature summary extracted from

  • Zheng, R.; Blanchard, J.S.
    Substrate specificity and kinetic isotope effect analysis of the Eschericia coli ketopantoate reductase (2003), Biochemistry, 42, 11289-11296.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.169 overexpression in strain BL21(DE3) Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.169 additional information
-
 additional information steady-state kinetics, pH-dependence of kinetics with different substrates, overview Escherichia coli
1.1.1.169 0.004
-
 beta-NADPH pH 7.5, 25°C, recombinant enzyme Escherichia coli
1.1.1.169 0.018
-
 thio-NADPH pH 7.5, 25°C, recombinant enzyme Escherichia coli
1.1.1.169 0.036
-
 alpha-NADPH pH 7.5, 25°C, recombinant enzyme Escherichia coli
1.1.1.169 0.075
-
 3'-NADPH pH 7.5, 25°C, recombinant enzyme Escherichia coli
1.1.1.169 0.12
-
 2-oxopantoate pH 7.5, 25°C, recombinant enzyme Escherichia coli
1.1.1.169 3.8
-
 3-methyl-2-oxo-n-valerate pH 7.5, 25°C, recombinant enzyme Escherichia coli
1.1.1.169 8.7
-
 2-oxoisovalerate pH 7.5, 25°C, recombinant enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.169 2-oxopantoate + NADPH Escherichia coli part of the pantothenate biosynthesis (R)-pantoate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.169 Escherichia coli
-
 gene panE
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.169 2-oxoisovalerate + NADPH low activity Escherichia coli 2-hydroxyvalerate + NADP+
-
 r
1.1.1.169 2-oxopantoate + 3'-NADPH
-
 Escherichia coli (R)-pantoate + 3'-NADP+
-
 r
1.1.1.169 2-oxopantoate + alpha-NADPH
-
 Escherichia coli (R)-pantoate + alpha-NADP+
-
 r
1.1.1.169 2-oxopantoate + beta-NADPH highly specific for Escherichia coli (R)-pantoate + beta-NADP+
-
 r
1.1.1.169 2-oxopantoate + NADPH part of the pantothenate biosynthesis Escherichia coli (R)-pantoate + NADP+
-
 ?
1.1.1.169 2-oxopantoate + thio-NADPH
-
 Escherichia coli (R)-pantoate + thio-NADP+
-
 r
1.1.1.169 3-methyl-2-oxo-n-valerate + NADPH low activity Escherichia coli 2-hydroxy-3-methyl-n-valerate + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.169 ketopantoate reductase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.169 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.169 7.5
-
 assay at Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.169 additional information
-
 pH-dependence of kinetics with different substrates, modeling, overview Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.169 3'-NADPH
-
 Escherichia coli
1.1.1.169 alpha-NADPH
-
 Escherichia coli
1.1.1.169 beta-NADPH preference for beta-NADPH as cofactor Escherichia coli
1.1.1.169 thio-NADPH
-
 Escherichia coli