EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.169 | overexpression in strain BL21(DE3) | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.169 | additional information | - |
additional information | steady-state kinetics, pH-dependence of kinetics with different substrates, overview | Escherichia coli | |
1.1.1.169 | 0.004 | - |
beta-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
1.1.1.169 | 0.018 | - |
thio-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
1.1.1.169 | 0.036 | - |
alpha-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
1.1.1.169 | 0.075 | - |
3'-NADPH | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
1.1.1.169 | 0.12 | - |
2-oxopantoate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
1.1.1.169 | 3.8 | - |
3-methyl-2-oxo-n-valerate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli | |
1.1.1.169 | 8.7 | - |
2-oxoisovalerate | pH 7.5, 25°C, recombinant enzyme | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.169 | 2-oxopantoate + NADPH | Escherichia coli | part of the pantothenate biosynthesis | (R)-pantoate + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.169 | Escherichia coli | - |
gene panE | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.169 | 2-oxoisovalerate + NADPH | low activity | Escherichia coli | 2-hydroxyvalerate + NADP+ | - |
r | |
1.1.1.169 | 2-oxopantoate + 3'-NADPH | - |
Escherichia coli | (R)-pantoate + 3'-NADP+ | - |
r | |
1.1.1.169 | 2-oxopantoate + alpha-NADPH | - |
Escherichia coli | (R)-pantoate + alpha-NADP+ | - |
r | |
1.1.1.169 | 2-oxopantoate + beta-NADPH | highly specific for | Escherichia coli | (R)-pantoate + beta-NADP+ | - |
r | |
1.1.1.169 | 2-oxopantoate + NADPH | part of the pantothenate biosynthesis | Escherichia coli | (R)-pantoate + NADP+ | - |
? | |
1.1.1.169 | 2-oxopantoate + thio-NADPH | - |
Escherichia coli | (R)-pantoate + thio-NADP+ | - |
r | |
1.1.1.169 | 3-methyl-2-oxo-n-valerate + NADPH | low activity | Escherichia coli | 2-hydroxy-3-methyl-n-valerate + NADP+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.169 | ketopantoate reductase | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.169 | 25 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.169 | 7.5 | - |
assay at | Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.169 | additional information | - |
pH-dependence of kinetics with different substrates, modeling, overview | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.169 | 3'-NADPH | - |
Escherichia coli | |
1.1.1.169 | alpha-NADPH | - |
Escherichia coli | |
1.1.1.169 | beta-NADPH | preference for beta-NADPH as cofactor | Escherichia coli | |
1.1.1.169 | thio-NADPH | - |
Escherichia coli |