BRENDA - Enzyme Database

Effect of AMP on mRNA binding by yeast NAD+-specific isocitrate dehydrogenase

Anderson, S.L.; Schirf, V.; McAlister-Henn, L.; Biochemistry 41, 7065-7073 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.41
AMP
activation of the enzyme and reduction of enzyme inhibition by mitochondrial mRNA, highly enhanced by presence of isocitrate, complex formation, overview
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
mRNA
enzyme specifically binds to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions allosterically inhibit the enzyme, which can be relieved by activator AMP in presence of isocitrate, complex formation, overview
Saccharomyces cerevisiae
1.1.1.42
additional information
activity is not affected by the nonspecific binding of the mitochondrial isozyme, not the cytosolic one, to 5'-untranslated regions of yeast mitochondrial mRNAs
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Saccharomyces cerevisiae
5739
-
1.1.1.42
cytosol
-
Saccharomyces cerevisiae
5829
-
1.1.1.42
mitochondrion
-
Saccharomyces cerevisiae
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mg2+
-
Saccharomyces cerevisiae
1.1.1.42
Mg2+
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Saccharomyces cerevisiae
enzyme has a regulatory role in the tricarboxylic cycle
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
additional information
Saccharomyces cerevisiae
allosteric regulation, enzyme specifically binds to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions allosterically inhibit the enzyme, which can be relieved by activator AMP in presence of isocitrate, complex formation, overview
?
-
-
-
1.1.1.42
isocitrate + NADP+
Saccharomyces cerevisiae
-
2-oxoglutarate + CO2 + NADPH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Saccharomyces cerevisiae
-
-
-
1.1.1.42
Saccharomyces cerevisiae
-
cytosolic isozyme IDP1, and mitochondrial isozyme IDP2
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
654652
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
enzyme has a regulatory role in the tricarboxylic cycle
654652
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
additional information
allosteric regulation, enzyme specifically binds to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions allosterically inhibit the enzyme, which can be relieved by activator AMP in presence of isocitrate, complex formation, overview
654652
Saccharomyces cerevisiae
?
-
-
-
-
1.1.1.42
D-isocitrate + NADP+
-
654652
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
654652
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADPH
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.4
-
assay at
Saccharomyces cerevisiae
1.1.1.42
7.8
-
assay at
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
dependent on
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.41
AMP
activation of the enzyme and reduction of enzyme inhibition by mitochondrial mRNA, highly enhanced by presence of isocitrate, complex formation, overview
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
dependent on
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
mRNA
enzyme specifically binds to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions allosterically inhibit the enzyme, which can be relieved by activator AMP in presence of isocitrate, complex formation, overview
Saccharomyces cerevisiae
1.1.1.42
additional information
activity is not affected by the nonspecific binding of the mitochondrial isozyme, not the cytosolic one, to 5'-untranslated regions of yeast mitochondrial mRNAs
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Saccharomyces cerevisiae
5739
-
1.1.1.42
cytosol
-
Saccharomyces cerevisiae
5829
-
1.1.1.42
mitochondrion
-
Saccharomyces cerevisiae
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mg2+
-
Saccharomyces cerevisiae
1.1.1.42
Mg2+
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Saccharomyces cerevisiae
enzyme has a regulatory role in the tricarboxylic cycle
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
additional information
Saccharomyces cerevisiae
allosteric regulation, enzyme specifically binds to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions allosterically inhibit the enzyme, which can be relieved by activator AMP in presence of isocitrate, complex formation, overview
?
-
-
-
1.1.1.42
isocitrate + NADP+
Saccharomyces cerevisiae
-
2-oxoglutarate + CO2 + NADPH
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
654652
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
enzyme has a regulatory role in the tricarboxylic cycle
654652
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
additional information
allosteric regulation, enzyme specifically binds to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions allosterically inhibit the enzyme, which can be relieved by activator AMP in presence of isocitrate, complex formation, overview
654652
Saccharomyces cerevisiae
?
-
-
-
-
1.1.1.42
D-isocitrate + NADP+
-
654652
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
654652
Saccharomyces cerevisiae
2-oxoglutarate + CO2 + NADPH
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.4
-
assay at
Saccharomyces cerevisiae
1.1.1.42
7.8
-
assay at
Saccharomyces cerevisiae