Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rydberg, E.H.; Li, C.; Maurus, R.; Overall, C.M.; Brayer, G.D.; Withers, S.G.
    Mechanistic analyses of catalysis in human pancreatic alpha-amylase: detailed kinetic and structural studies of mutants of three conserved carboxylic acids (2002), Biochemistry, 41, 4492-4502.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Pichia pastoris Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.1
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.1 D197A no activity Homo sapiens
3.2.1.1 D197N no activity Homo sapiens
3.2.1.1 D300A 0.0005% of wild-type starch hydrolyzing activity Homo sapiens
3.2.1.1 D300N 0.0005% of wild-type starch hydrolyzing activity Homo sapiens
3.2.1.1 E233A 0.005% of wild-type starch hydrolyzing activity Homo sapiens
3.2.1.1 E233A/D300A 0.001% of wild-type starch hydrolyzing activity Homo sapiens
3.2.1.1 E233Q 0.0005% of wild-type starch hydrolyzing activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 0.51
-
 alpha-maltotriosyl fluoride 30°C, pH 7.0 Homo sapiens
3.2.1.1 4.5
-
 alpha-maltosyl fluoride 30°C, pH 7.0 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 56091
-
 x * 56091, deduced from nucloeotide sequence Homo sapiens
3.2.1.1 56095
-
 x * 56095, mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.1 starch + H2O Homo sapiens
-
 maltooligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 recombinant alpha-amylase, Phenyl Sepharose, Q-Sepharose Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.1 pancreas
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 5500
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 alpha-maltosyl fluoride + H2O
-
 Homo sapiens maltose + fluoride
-
 ?
3.2.1.1 alpha-maltotriosyl fluoride + H2O
-
 Homo sapiens maltotriose + fluoride
-
 ?
3.2.1.1 maltopentaose + H2O
-
 Homo sapiens maltotriose + maltose major products ?
3.2.1.1 starch + H2O
-
 Homo sapiens maltooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 56091, deduced from nucloeotide sequence Homo sapiens
3.2.1.1 ? x * 56095, mass spectrometry Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 HPA
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 283
-
 alpha-maltotriosyl fluoride 30°C, pH 7.0 Homo sapiens
3.2.1.1 443
-
 alpha-maltosyl fluoride 30°C, pH 7.0 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 7 8.5 hydrolysis of alpha-maltotriosyl fluoride Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 5.5 10
-
 Homo sapiens