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Literature summary extracted from

  • Horswill, A.R.; Escalante-Semerena, J.C.
    Characterization of the propionyl-CoA synthetase (PrpE) enzyme of Salmonella enterica: residue Lys592 is required for propionyl-AMP synthesis (2002), Biochemistry, 41, 2379-2387.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.2.1.17 synthesis used for incorporation of unusual acyl groups into biopolymers and polyketide antibiotics Salmonella enterica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.17 expressed in Escherichia coli ER2566 Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.17 G245A 58% of activity Salmonella enterica
6.2.1.17 G249A 11% of activity Salmonella enterica
6.2.1.17 K248A 12% of activity Salmonella enterica
6.2.1.17 K248E 6% of activity Salmonella enterica
6.2.1.17 K592A complete loss of activity Salmonella enterica
6.2.1.17 K592E complete loss of activity Salmonella enterica
6.2.1.17 P247A activity almost not affected Salmonella enterica

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.17 adenosine 5'-propyl phosphate strong inhibition, bi uni uni bi ping pong mechanism Salmonella enterica
6.2.1.17 propyl-AMP competitive with ATP and propionyl-AMP, mixed type inhibition with propionate, uncompetitive with CoA Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.17 0.02
-
 propionate pH 7.5, 37°C Salmonella enterica
6.2.1.17 0.023
-
 propionyl-AMP pH 7.5, 37°C Salmonella enterica
6.2.1.17 0.057
-
 ATP pH 7.5, 37°C Salmonella enterica
6.2.1.17 0.215
-
 CoA pH 7.5, 37°C Salmonella enterica
6.2.1.17 0.254
-
 diphosphate pH 7.5, 37°C Salmonella enterica
6.2.1.17 0.279
-
 Acrylate pH 7.5, 37°C Salmonella enterica
6.2.1.17 1.7
-
 acetate pH 7.5, 37°C Salmonella enterica
6.2.1.17 5.6
-
 Butyrate pH 7.5, 37°C Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.17 ATP + propanoate + CoA Salmonella enterica required for propionate catabolism AMP + diphosphate + propanoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.17 Salmonella enterica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.17 native and mutant enzymes using chitin affinity chromatography Salmonella enterica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.17 0.06
-
 native enzyme with glycine as substrate, pH 7.5, 37°C Salmonella enterica
6.2.1.17 0.65
-
 native enzyme with glyoxalate as substrate, pH 7.5, 37°C Salmonella enterica
6.2.1.17 18
-
 native enzyme with mercaptoacetoacetate as substrate, pH 7.5, 37°C Salmonella enterica
6.2.1.17 22
-
 native enzyme with propionate as substrate, pH 7.5, 37°C Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.17 ATP + acetate + CoA very poor substrate Salmonella enterica AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.17 ATP + acrylate + CoA poor substrate Salmonella enterica AMP + diphosphate + acryloyl-CoA
-
 ?
6.2.1.17 ATP + butyrate + CoA very poor substrate Salmonella enterica AMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.17 ATP + glycine + CoA very poor substrate Salmonella enterica ?
-
 ?
6.2.1.17 ATP + glyoxalate + CoA poor substrate Salmonella enterica ?
-
 ?
6.2.1.17 ATP + mercaptoacetoacetate + CoA very good substrate Salmonella enterica AMP + diphosphate + mercaptoacetoacetyl-CoA
-
 ?
6.2.1.17 ATP + propanoate + CoA required for propionate catabolism Salmonella enterica AMP + diphosphate + propanoyl-CoA
-
 ?
6.2.1.17 ATP + propanoate + CoA via propionyl-AMP intermediate Salmonella enterica AMP + diphosphate + propanoyl-CoA
-
 ?
6.2.1.17 propionyl-AMP + CoA
-
 Salmonella enterica AMP + propanoyl-CoA
-
 ir

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.17 6
-
 Butyrate pH 7.5, 37°C Salmonella enterica
6.2.1.17 26
-
 Acrylate pH 7.5, 37°C Salmonella enterica
6.2.1.17 32
-
 acetate pH 7.5, 37°C Salmonella enterica
6.2.1.17 33
-
 propionate pH 7.5, 37°C Salmonella enterica
6.2.1.17 34
-
 ATP pH 7.5, 37°C Salmonella enterica
6.2.1.17 42
-
 CoA pH 7.5, 37°C Salmonella enterica
6.2.1.17 78
-
 propionyl-AMP pH 7.5, 37°C Salmonella enterica
6.2.1.17 79
-
 diphosphate pH 7.5, 37°C Salmonella enterica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.2.1.17 0.00002
-
 propyl-AMP competitive with ATP, pH 7.5, 37°C Salmonella enterica